Deletions in the 7a ORF of feline coronavirus associated with an epidemic of feline infectious peritonitis
Deletions in the 7a ORF of feline coronavirus associated with an epidemic of feline infectious peritonitis
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DOI：
10.1016/s0378-1135(01)00354-6
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发表时间：
2001-08-08
影响因子：
3.3
通讯作者：
Kania, S
中科院分区：
文献类型：
--
作者：
Kennedy, M;Boedeker, N;Kania, S
一群波斯猫在2年多的时间里经历了猫传染性腹膜炎（FIP）的流行。在此期间出生的幼崽中发生了12例FIP。感染FIP的猫都是通过父系遗传的。本研究在成年猫、患FIP的小猫及其兄弟姐妹的生物样本中一致检测到猫冠状病毒（FCoV）基因组RNA。对病毒7a/7b开放阅读框（ORF）进行分析，发现在人群中流行的两种不同的病毒变体，一种具有完整的7a ORF，另一种在7a ORF中有两个主要缺失。7b ORF在所有病毒分离株中都是完整且相似的，尽管存在导致氨基酸改变的点突变。父系被确定感染了这两种变异，并且持续受到病毒感染。我们推测，在这个群体中，可能在父系中，缺失变异是由病毒复制过程中的非缺失变异引起的。(C) 2001 Elsevier Science B.V.版权所有
A population of Persian cats experienced an epidemic of feline infectious peritonitis (FIP) over 2 years. Twelve cases of FIP occurred in litters born during this period. Cats contracting FIP were all genetically related through the sire. Feline coronavirus (FCoV) genomic RNA was detected consistently in this study in biologic samples from adult cats, kittens suffering from FIP, and their siblings. Analysis of viral 7a/7b open reading frame (ORFs) were analyzed and revealed two distinct virus variants circulating in the population, one with an intact 7a ORF and one with two major deletions in the 7a ORE The 7b ORFs were intact and similar among all virus isolates, although point mutations resulting in amino acid changes were present. The sire was determined to be infected with both variants, and was persistently virus-infected. We speculate the deletion variant arose from the non-deletion variant during viral replication in this population, possibly in the sire. (C) 2001 Elsevier Science B.V. All rights reserved.