First blood vessels in the avian neural tube are formed by a combination of dorsal angioblast immigration and ventral sprouting of endothelial cells
First blood vessels in the avian neural tube are formed by a combination of dorsal angioblast immigration and ventral sprouting of endothelial cells
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DOI：
10.1006/dbio.1996.0012
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发表时间：
1996-01-10
影响因子：
2.7
通讯作者：
Christ, B
中科院分区：
文献类型：
--
作者：
Kurz, H;Gartner, T;Christ, B
我们研究了鹌鹑胚胎和鸡-鹌鹑嵌合体神经管（NT）血管化的早期模式。在Hamburger和汉密尔顿（HH）第19期，成血管细胞首先出现在背侧三分之一处，为单个迁移细胞。它们的分布不符合分段模式。在这种初始的背侧迁移后，内皮芽侵入底板两侧的NT（HH阶段21）。这些细胞与它们的动脉血管源保持连续，在HH-阶段22连接到静脉神经周围血管丛，并在HH-阶段23形成NT的第一灌注血管。在从菱脑NT尾侧开始的头尾序列中观察到相同的血管营养性血管形成模式。在萌发细胞上观察到极长的丝状伪足，向中央管和套层延伸。鸡-鹌鹑嵌合体证明了血管母细胞的完全神经外胚层起源。用鸡NT移植物替代鹌鹑NT后，嵌合体中成血管细胞和芽的分布与对照组相同。我们的结论是NT接收它的第一个血管由两个不同的过程，孤立的迁移成血管细胞和腹侧发芽的内皮细胞，它来自灌注血管的背侧移民的组合。背侧侵入性成血管细胞在穿过神经管后有助于神经内血管丛的发育。神经上皮内血管的初始分布与成血管细胞的内在随机运动相对应;稍后可以看到更规则的模式。地板显然禁止在NT两侧的芽之间的连接，而在背NT，这样的分离效果的迁移成血管细胞不存在。(C)出版社：Academic Press，Inc.
We studied the early pattern of neural tube (NT) vascularization in quail embryos and chick-quail chimeras. Angioblasts appeared first in the dorsal third at Hamburger and Hamilton (HH) stage 19 as single, migrating cells. Their distribution did not correspond to a segmental pattern. After this initial dorsal immigration, endothelial sprouts invaded the NT on either side of the floor plate (HH stage 21). These cells remained continuous with their arterial vascular sources, connected to the venous perineural vascular plexus at HH-stage 22, and formed the first perfused vessels of the NT at HH-stage 23. The same pattern of angiotrophic vascularization was observed in a craniocaudal sequence starting caudal to the rhombencephalic NT. Extremely long filopodia were observed on sprouting cells, extending toward the central canal and the mantle layer. The exclusively extraneuroectodermal origin of angioblastic cells was demonstrated with chick-quail chimeras. Following replacement of quail NT by chick NT graft, angioblast and sprout distribution in chimeras was the same as in controls. We conclude that the NT receives its first blood vessels by a combination of two different processes, dorsal immigration of isolated migrating angioblastic cells and ventral sprouting of endothelial cells, which derive from perfused vessels. The dorsal invasive angioblasts contribute to the developing intraneural vascular plexus after having traversed the neural tube. The initial distribution of blood vessels within the neuroepithelium corresponds to intrinsic random motility of angioblastic cells; a more regular pattern is seen later. The floor plate apparently prohibits connections between sprouts in both NT sides, whereas in the dorsal NT, such a separating effect on the migrating angioblasts does not exist. (C) 1996 Academic Press, Inc.