FLImBrush: dynamic visualization of intraoperative free-hand fiber-based fluorescence lifetime imaging
FLImBrush: dynamic visualization of intraoperative free-hand fiber-based fluorescence lifetime imaging
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DOI：
10.1364/boe.398357
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发表时间：
2020-09-01
影响因子：
3.4
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Marsden, Mark;Fukazawa, Takanori;Marcu, Laura
医学成像的徒手扫描方法允许灵活、轻便的探头对复杂的解剖结构进行成像，用于荧光寿命成像（FLIm）、光学相干断层扫描（OCT）和超声等模式。虽然非常有前途，但这种方法面临着几个关键挑战，包括成像期间的组织运动、手术野中的不同照明条件以及组织表面的稀疏采样。这些挑战限制了所采集的成像数据的配准准确性和可解释性。在这里，我们报告FLImBrush作为一个强大的方法，术中徒手光纤荧光寿命成像（FLIm）的定位和可视化。FLImBrush建立在现有方法的基础上，同时采用基于深度学习的图像分割，基于块匹配的运动校正和基于插值的可视化来解决上述挑战。目前的结果表明，FLImBrush可以提供FLIm点测量的准确定位，同时产生从组织表面采集的FLIm数据的可解释和完整的可视化。每个主要处理步骤都能够实时处理（每秒> 30帧），突出了FLImBrush用于术中成像和手术指导的可行性。目前的研究结果表明，将FLImBrush整合到一系列手术应用中是可行的，包括头颈部手术期间的癌症边缘评估。(C)根据OSA开放获取出版协议的条款，2020年美国光学学会
A free-hand scanning approach to medical imaging allows for flexible, lightweight probes to image intricate anatomies for modalities such as fluorescence lifetime imaging (FLIm), optical coherence tomography (OCT) and ultrasound. While very promising, this approach faces several key challenges including tissue motion during imaging, varying lighting conditions in the surgical field, and sparse sampling of the tissue surface. These challenges limit the coregistration accuracy and interpretability of the acquired imaging data. Here we report FLImBrush as a robust method for the localization and visualization of intraoperative free-hand fiber optic fluorescence lifetime imaging (FLIm). FLImBrush builds upon an existing method while employing deep learning-based image segmentation, block-matching based motion correction, and interpolation-based visualization to address the aforementioned challenges. Current results demonstrate that FLImBrush can provide accurate localization of FLIm point-measurements while producing interpretable and complete visualizations of FLIm data acquired from a tissue surface. Each of the main processing steps was shown to be capable of real-time processing (> 30 frames per second), highlighting the feasibility of FLImBrush for intraoperative imaging and surgical guidance. Current findings show the feasibility of integrating FLImBrush into a range of surgical applications including cancer margins assessment during head and neck surgery. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement