Microimaging characterization of a B16-F10 melanoma metastasis mouse model
Microimaging characterization of a B16-F10 melanoma metastasis mouse model
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DOI：
10.2310/7290.2006.00011
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发表时间：
2006-04-01
影响因子：
2.8
通讯作者：
Hoffman, Timothy J.
中科院分区：
文献类型：
--
作者：
Winkelmann, Christopber T.;Figueroa, Said Daibes;Hoffman, Timothy J.
转移性黑色素瘤小鼠模型的特征在于大体尸检检查、组织病理学和光学成像。为了确定黑色素瘤转移的时间进展、程度和代谢是否可以非侵入性地监测，通过使用黑色素瘤小鼠模型进行了系列微CT和小动物PET成像研究。向幼年雌性C57 BL/6小鼠静脉内注射同系B16-F10黑素瘤细胞。对麻醉小鼠进行系列显微CT成像研究。在出现不良临床体征时或注射后第30天对小鼠进行尸检，并采集组织进行组织病理学检查。在四只小鼠的单独研究中，用2-脱氧-2-[F-18]氟-D-葡萄糖（[F-18]FDG）评估肿瘤活力，并通过使用小动物PET成像进行研究。总共有59%的小鼠发生了转移性肿瘤。Micro-CT图像分析能够识别并随访高达36%的转移性病变。通过显微CT成像识别和随访的转移性病变的实例包括肺转移、下颌转移、皮下转移和胫骨/股骨转移。显微CT和小动物PET融合成像成功地将转移性肿瘤的葡萄糖代谢的解剖定位相关联。发现微CT和小动物PET成像在检测和表征由该转移性黑色素瘤模型产生的病变方面非常有效。
Metastatic mouse models of melanoma have been characterized by gross necropsy examination, histopathology, and optical imaging. To determine if the time progression, extent, and metabolism of melanoma metastases could be monitored noninvasively, serial micro-CT and small-animal PET imaging studies were performed by using a mouse model of melanoma. juvenile female C57BL/6 mice were injected intravenously with syngenic B16-F10 melanoma cells. Serial micro-CT imaging studies were performed on anesthetized mice. Mice were necropsied at the development of adverse clinical signs or at postinjection Day 30, and tissues were collected for histopathology. In a separate study of four mice, tumor viability was assessed with 2-deox)-2-[F-18]fluoro-D-glucose ([F-18]FDG) and studied by using small-animal PET imaging. A total of 59% of the mice developed metastatic tumors. Micro-CT image analysis was able to identify and follow up to 36% of metastatic lesions. Examples of metastatic lesions identified and followed up by micro-CT imaging included a lung metastasis, mandibular metastasis, subcutaneous metastasis, and tibial/femoral metastasis. Micro-CT and small-animal PET fusion imaging successfully correlated anatomic localization of glucose metabolism of the metastatic tumors. Micro-CT and small-animal PET imaging were found to be highly effective in detection and characterization of lesions produced by this metastatic melanoma model.