INCREASE OF GANGLIOSIDES IN ATHEROSCLEROTIC HUMAN AORTAS
INCREASE OF GANGLIOSIDES IN ATHEROSCLEROTIC HUMAN AORTAS
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DOI：
10.1007/bf02532490
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发表时间：
1975-01-01
期刊：
影响因子：
1.9
通讯作者：
SILVER, MD
中科院分区：
文献类型：
--
作者：
BRECKENRIDGE, WC;HALLORAN, JL;SILVER, MD
在人死后获得含有内膜和介质的人胸主动脉的制备，并通过标准技术分离神经节苷脂。通过气相液相色谱法测定，神经节苷脂唾液酸的含量在正常主动脉中最低（33±9 nmol N -乙酰神经氨酸/g湿组织），在主要含有脂肪条纹的主动脉中逐渐增加（54±1 nmol N -乙酰神经氨酸/g湿组织），升高的黄色斑块（88±23 nmol N -乙酰神经氨酸/g湿组织），溃疡病变（270±44 nmol N -乙酰神经氨酸/h湿组织）。神经节苷脂的薄层色谱和组成糖的气相液相色谱都证明了神经节苷脂的存在，其性质类似于单谷糖基神经酰胺（GM3）作为主要的神经节苷脂。从血浆和低密度脂蛋白中分离出具有相似化学特性的神经节苷脂。
Preparations of human thoracic aortas containing intima and media were obtained post‐mortem, and gangliosides were isolated by standard techniques. The quantity of ganglioside sialic acid, as assayed by gas liquid chromatography, was lowest in normal aortas (33±9 nmoles N‐acetyl neuraminic acid/g wet tissue) and progressively increased in aortas containing predominantly fatty streaks (54±1 nmoles N‐acetyl neuraminic acid/g wet tissue), raised yellow plaques (88±23 nmoles N‐acetyl neuraminic acid/g wet tissue), and ulcerated lesions (270±44 nmoles N‐acetyl neuraminic acid/h wet tissues). Both thin layer chromatography of the gangliosides and gas liquid chromatography of the constituent sugars demonstrated the presence of a ganglioside with properties similar to a monosialyl lactosyl ceramide (GM3) as the major ganglioside. A ganglioside with similar chemical characteristics was isolated from plasma and low density lipoproteins.