Vasoactive intestinal peptide (VIP) induces malignant transformation of the human prostate epithelial cell line RWPE-1
Vasoactive intestinal peptide (VIP) induces malignant transformation of the human prostate epithelial cell line RWPE-1
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DOI：
10.1016/j.canlet.2010.07.019
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发表时间：
2010-12-18
期刊：
影响因子：
9.7
通讯作者：
Carmena, Maria J.
中科院分区：
文献类型：
--
作者：
Fernandez-Martinez, Ana B.;Bajo, Ana M.;Carmena, Maria J.
在非肿瘤人前列腺上皮细胞（RWPE-1）和体内异种移植物中分析了血管活性肠肽（VIP）的致癌潜力，VIP诱导的形态学变化和迁移表型与刺激金属蛋白酶MMP-2和MMP-9的表达/活性一致，降低了E钙粘蛋白介导的细胞增殖。细胞粘附和增加细胞运动性VIP增加细胞周期蛋白D1表达和细胞增殖，在VPAC（1）-受体siRNA转染后被阻断。1通过皮下注射VIP处理的细胞在无胸腺裸鼠中形成的肿瘤VIP在RWPE 1细胞转化中作为细胞因子，可以想象通过上皮-间充质转化（EMT）加强VIP在前列腺肿瘤发生中的作用（C）2010 Elsevier爱尔兰有限公司版权所有
The carcinogenic potential of vasoactive intestinal peptide (VIP) was analyzed in non-tumor human prostate epithelial cells (RWPE-1) and in vivo xenografts VIP induced morphological changes and a migratory phenotype consistent with stimulation of expression/activity of metalloproteinases MMP-2 and MMP 9 decreased E cadherin-mediated cell-cell adhesion and increased cell motility VIP increased cyclin D1 expression and cell proliferation that was blocked after VPAC(1)-receptor siRNA transfection Similar effects were seen in RWPE-1 tumors developed by subcutaneous injection of VIP-treated cells in athymic nude mice VIP acts as a cytokine in RWPE 1 cell transformation conceivably through epithelial-mesenchymal transition (EMT) reinforcing VIP role in prostate tumorigenesis (C) 2010 Elsevier Ireland Ltd All rights reserved