DNA sequence and genome organization of genital human papillomavirus type 6b.
DNA sequence and genome organization of genital human papillomavirus type 6b.
复制标题
生殖器人乳头瘤病毒 6b 型的 DNA 序列和基因组组织。
作者：
E. Schwarz;M. Dürst;C. Demankowski;O. Lattermann;R. Zech;E. Wolfsperger;S. Suhai;H. Hausen
测定了生殖器人乳头瘤病毒6b型（HPV6b）环状双链DNA的完整核苷酸序列（7902 bp），并与人乳头瘤病毒1a型（HPV1a）和牛乳头瘤病毒1型（BPV1）的DNA序列进行了比较。所有主要的开放阅读框仅位于一条DNA链上。它们的排列显示HPV 6b的基因组结构与HPV 1a和BPV1相似。推定的早期区域包括两个大的开放阅读框E1和E2，其与HPV 1a和BPV 1具有显著的氨基酸序列同源性，其侧翼是几个较小的框。E2的内部部分与另一开放阅读框架E4完全重叠。推定的晚期区域包含两个大的开放阅读框L1和L2。L1氨基酸序列在分析的乳头瘤病毒类型中高度保守。通过序列比较，潜在的启动子，剪接和多聚腺苷酸化信号可以定位在HPV 6b DNA提示生殖器乳头瘤病毒基因表达的可能机制。
The complete nucleotide sequence of the circular double‐stranded DNA of the genital human papillomavirus type 6b (HPV6b) comprising 7902 bp was determined and compared with the DNA sequences of human papillomavirus type 1a (HPV1a) and bovine papillomavirus type 1 (BPV1). All major open reading frames are located on one DNA strand only. Their arrangement reveals that the genomic organization of HPV6b is similar to that of HPV1a and BPV1. The putative early region includes two large open reading frames E1 and E2 with marked amino acid sequence homologies to HPV1a and BPV1 which are flanked by several smaller frames. The internal part of E2 completely overlaps with another open reading frame E4. The putative late region contains two large open reading frames L1 and L2. The L1 amino acid sequences are highly conserved among analyzed papillomavirus types. By sequence comparison, potential promoter, splicing and polyadenylation signals can be localized in HPV6b DNA suggesting possible mechanisms of genital papillomavirus gene expression.