IFN-gamma and IL-12 are increased in active compared with inactive tuberculosis
IFN-gamma and IL-12 are increased in active compared with inactive tuberculosis
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DOI：
10.1164/ajrccm.155.3.9116999
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发表时间：
1997-03-01
影响因子：
24.7
通讯作者：
Hamid, Q
中科院分区：
文献类型：
--
作者：
Taha, RA;Kotsimbos, TC;Hamid, Q
细胞因子介导的对结核分枝杆菌感染的免疫应答是结核分枝杆菌疾病发展和病理的重要决定因素。然而，结核分枝杆菌感染引起的细胞因子谱变化与活动性肺结核相关的细胞因子谱变化之间的区别尚不清楚。我们比较了活动性肺结核患者和非活动性肺结核患者支气管肺泡灌洗（BAL）中的T细胞及其亚群、巨噬细胞和细胞因子信使RNA （mRNA）谱。10例微生物学证实的活动性肺结核患者和25例非活动性肺结核患者被招募。所有病例均进行BAL支气管镜检查，并使用免疫细胞化学和原位杂交技术检测BAL细胞自旋。与非活动性结核相比，活动性结核中CD8(+) T细胞的BAL细胞百分比显著增加（平均+/- SEM: 7.2 +/- 0.9 vs 2.1 +/- 0.4, p < 0.001），但CD3（+）或CD4(+) T细胞和巨噬细胞没有增加。活动性肺结核患者与非活动性肺结核患者中表达干扰素- γ (ifn - γ)和白细胞介素-12 (IL-12) mRNA的BAL细胞百分比显著增加（分别为8.0 +/- 0.6 vs 3.7 +/- 0.4和28.4 +/- 2.3 vs 10.2 +/- 1.0, p < 0.001）。活性组和非活性组表达IL-2、肿瘤坏死因子- α (tnf - α)、IL-4和IL-5 mRNA的细胞数量无显著差异。总之，活动性肺结核与BAL中CD8（+）细胞数量的增加以及IL-12和ifn - γ mRNA表达的显著增加有关，这两者都可能是疾病活动性的有用标志物。
Cytokine-mediated immune responses to Mycobacterium tuberculosis infection are important determinants of M. tuberculosis disease development and pathology. However, the distinction between changes in cytokine profile attributable to M. tuberculosis infection and those associated with active pulmonary tuberculosis is unclear. We have compared T cells and their subsets, macrophages, and cytokine messenger RNA (mRNA) profile in the bronchoalveolar lavage (BAL) of patients with active pulmonary tuberculosis with inactive tuberculosis subjects. Ten patients with microbiologically confirmed active pulmonary tuberculosis and 25 subjects with inactive tuberculosis were recruited. Bronchoscopy with BAL was undertaken in all cases and BAL cytospins were examined using the techniques of immunocytochemistry and in situ hybridization. There was a significant increase in the percentage of BAL cells that were CD8(+) T cells in active tuberculosis compared with inactive tuberculosis (mean +/- SEM: 7.2 +/- 0.9 versus 2.1 +/- 0.4, p < 0.001), but not CD3(+) or CD4(+) T cells nor macrophages. There were significant increases in the percentage of BAL cells expressing mRNA for interferon-gamma (IFN-gamma) and interleukin-12 (IL-12) in active versus inactive pulmonary tuberculosis subjects (8.0 +/- 0.6 versus 3.7 +/- 0.4 and 28.4 +/- 2.3 versus 10.2 +/- 1.0, p < 0.001, respectively). There were no significant differences between the active and inactive groups in the number of cells expressing mRNA for IL-2, tumor necrosis factor-alpha (TNF-alpha), IL-4, and IL-5. In conclusion, active pulmonary tuberculosis is associated with increased numbers of CD8(+) cells and marked increases in the expression of IL-12 and IFN-gamma mRNA in the BAL, both of which may be useful markers of disease activity.