Spiral waves in disinhibited mammalian neocortex
Spiral waves in disinhibited mammalian neocortex
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DOI：
10.1523/jneurosci.2705-04.2004
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发表时间：
2004-11-03
影响因子：
5.3
通讯作者：
Wu, JY
中科院分区：
文献类型：
--
作者：
Huang, XY;Troy, WC;Wu, JY
螺旋波是可激发系统的基本特征。虽然在各种生物系统中已经观察到了这种波，但在哺乳动物的大脑皮层中，神经元活动期间还没有观察到这种波。在这里，我们报告了电压敏感染料成像显示的大鼠新皮质脑片中稳定的旋转螺旋波。取自枕叶皮质(视觉)的组织进行平行于皮质层的切片，以保留III-V层(500微米厚，类似于4×6 mm(2))的水平连接。在这种切向切片中，激发波在胆碱能振荡过程中以二维方式传播。螺旋波是自发产生的，与平面波、环形波和不规则波交替出现。螺旋线的自转速度类似于每秒10圈，自转以一周一转的方式与振荡联系在一起。一个小的(
Spiral waves are a basic feature of excitable systems. Although such waves have been observed in a variety of biological systems, they have not been observed in the mammalian cortex during neuronal activity. Here, we report stable rotating spiral waves in rat neocortical slices visualized by voltage-sensitive dye imaging. Tissue from the occipital cortex (visual) was sectioned parallel to cortical lamina to preserve horizontal connections in layers III-V (500-mum-thick, similar to4 x 6 mm(2)). In such tangential slices, excitation waves propagated in two dimensions during cholinergic oscillations. Spiral waves occurred spontaneously and alternated with plane, ring, and irregular waves. The rotation rate of the spirals was similar to10 turns per second, and the rotation was linked to the oscillations in a one-cycle-one-rotation manner. A small (