Diet-induced ketosis increases monocarboxylate transporter (MCT1) levels in rat brain
Diet-induced ketosis increases monocarboxylate transporter (MCT1) levels in rat brain
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DOI：
10.1016/s0197-0186(00)00102-9
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发表时间：
2001-05-01
影响因子：
4.2
通讯作者：
Drewes, LR
中科院分区：
文献类型：
--
作者：
Leino, RL;Gerhart, DZ;Drewes, LR
采用光镜和电镜免疫细胞化学方法研究了高脂（生酮）饮食的成年Long-Evans大鼠脑中单羧酸转运体（MCT 1）的水平。与标准饮食大鼠相比，给予生酮饮食（91%脂肪和9%蛋白质）长达6周的大鼠脑内皮细胞和神经元中单羧酸转运蛋白MCT 1（和葡萄糖转运蛋白GLUT 1）水平升高。在酮血症大鼠中，电子显微镜免疫金方法显示，4周时脑内皮细胞中的MCT 1标记增加了8倍。腔外内皮细胞膜的两倍重标记的管腔膜。在控制中。管腔标记和管腔外标记没有显著差异。内皮细胞胞质区室被稀疏标记（
Monocarboxylate transporter (MCT1) levels in brains of adult Long-Evans rats on a high-fat (ketogenic) diet were investigated using light and electron microscopic immunocytochemical methods. Rats given the ketogenic diet (91% fat and 9% protein) for up to 6 weeks had increased levels of the monocarboxylate transporter MCT1 (and of the glucose transporter GLUT1) in brain endothelial cells and neuropil compared to rats on a standard diet. In ketonemic rats, electron microscopic immunogold methods revealed an 8-fold greater MCT1 labeling in the brain endothelial cells at 4 weeks. Abluminal endothelial membranes were twice as heavily labeled as luminal membranes. In controls. luminal and abluminal labeling was not significantly different. The endothelial cytoplasmic compartment was sparsely labeled (