Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase family genes in early mouse embryogenesis
Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase family genes in early mouse embryogenesis
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DOI：
10.1016/j.gep.2013.06.003
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发表时间：
2013-12-01
影响因子：
1.2
通讯作者：
Delgado-Olguin, Paul
中科院分区：
文献类型：
--
作者：
Chi, Lijun;Delgado-Olguin, Paul
包含 NOL1/NOP2/sun 结构域的基因编码 RNA 甲基转移酶 Nsun2、3、4、5、6 和 7。甲基化 RNA 遍布转录组，但人们对 RNA 甲基转移酶的功能知之甚少。 Nsun2和Nsun4参与细胞增殖和分化、蛋白质生物合成和癌症。此外，Nsun2和Nsun7功能障碍可能分别导致智力障碍和男性不育。 Nsun3、Nsun5 和 Nsun6 的功能未知。鉴于RNA甲基化的广泛分布，Nsun基因有可能参与更广泛的相关生物过程，包括胚胎发生的调节。在这里，我们描述了小鼠胚胎发育过程中 Nsun 基因的表达模式。原位杂交显示 Nsun 基因表达受发育调节。 Nsun 基因在原肠胚形成期间广泛表达，但随着胚胎发生的进行在特定组织中丰富。 Nsun 转录本在发育中的大脑中丰富，与神经认知发育中的拟议功能一致。此外，Nsun 转录本在发育中的耳、眼、嗅上皮、鳃弓、心脏和四肢中丰富，表明 NSUN 蛋白在神经、颅面、心脏和四肢形态发生中可能具有重叠功能。此外，Nsun2 和 Nsun6 在尾神经管和新形成的体节中富集，表明可能在身体轴延伸中发挥作用。这些结果表明 NSUN 蛋白和 RNA 甲基化在胚胎发育的广泛方面可能存在重叠功能。 (C) 2013 Elsevier B.V. 保留所有权利。
The NOL1/NOP2/sun domain-containing genes encode the RNA methyltransferases Nsun2, 3, 4, 5, 6 and 7. Methylated RNA pervades the transcriptome, yet the function of RNA methyltransferases is poorly understood. Nsun2 and Nsun4 participate in cell proliferation and differentiation, protein biosynthesis and cancer. In addition, Nsun2 and Nsun7 dysfunction might cause intellectual disability and male sterility, respectively. The functions of Nsun3, Nsun5 and Nsun6 are unknown. Given the widespread distribution of RNA methylation, it is possible that Nsun genes participate in a broader range of relevant biological processes including the regulation of embryogenesis. Here, we describe the expression pattern of Nsun genes during mouse embryo development. In situ hybridization showed developmentally regulated Nsun gene expression. Nsun genes express broadly during gastrulation, but enrich in specific tissues as embryogenesis proceeds. Nsun transcripts enrich in the developing brain, consistent with proposed functions in neurocognitive development. In addition, Nsun transcripts enrich in the developing ear, eye, olfactory epithelium, branchial arches, heart and limb, suggesting possible overlapping functions of NSUN proteins in neural, craniofacial, cardiac, and limb morphogenesis. Furthermore, Nsun2 and Nsun6 enrich in the caudal neural tube and newly formed somites, suggesting possible functions in body axis extension. These results suggest possible overlapping functions of NSUN proteins and RNA methylation in broad aspects of embryonic development. (C) 2013 Elsevier B.V. All rights reserved.