Nucleolus Precursor Body (NPB): A Distinct Structure in Mammalian Oocytes and Zygotes
Nucleolus Precursor Body (NPB): A Distinct Structure in Mammalian Oocytes and Zygotes
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DOI：
10.4161/19491034.2014.990858
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发表时间：
2014-11-01
期刊：
影响因子：
3.7
通讯作者：
Miyano, Takashi
中科院分区：
文献类型：
--
作者：
Kyogoku, Hirohisa;Kitajima, Tomoya S.;Miyano, Takashi
哺乳动物卵母细胞和受精卵中的核仁，有时称为核仁前体 (NPB)，结构紧凑，形态上与体细胞中的核仁不同。我们对受精卵应用了独特的 NPB 分析方法去核技术，以去除 NPB。据报道，卵母细胞 NPB 对胚胎发育至关重要；如果没有它们，卵母细胞会完全成熟并可以受精，但受精卵和胚胎中不会形成核仁，导致发育失败。然而，我们发现，当从受精卵中去除 NPB 时，受精卵成功发育成活产幼崽。这些结果表明卵母细胞 NPB 对胚胎发育至关重要，但受精卵 NPB 则不然。此外，去核的受精卵在早期胚胎发育过程中形成了体细胞型核仁，这表明体细胞型核仁并非源自受精卵NPB。我们总结了最近对 NPB 的调查，并提供了更多评论和调查结果。
Nucleoli in mammalian oocytes and zygotes, sometimes referred to as nucleolus precursor bodies (NPBs), are compact and morphologically different from nucleoli in somatic cells. We applied a unique NPB analyzing method enucleolation technique to zygotes to remove the NPBs. It has been reported that oocyte NPBs are essential for embryonic development; in their absence, the oocytes complete maturation and can be fertilized, but no nucleoli are formed in the zygotes and embryos, leading to developmental failure. However, we found that when NPBs were removed from zygotes, the zygotes developed successfully to live-born pups. These results indicated that oocyte NPBs are essential for embryonic development, but zygote NPBs are not. In addition, the enucleolated zygotes formed somatic-type nucleoli during early embryonic development, demonstrating that somatic-type nucleoli do not originate from zygote NPBs. We summarize our recent investigation on NPBs, and provide additional comments and findings.