PROTECTION OF MONKEYS AGAINST EXPERIMENTAL SHIGELLOSIS WITH A LIVING ATTENUATED ORAL POLYVALENT DYSENTERY VACCINE
PROTECTION OF MONKEYS AGAINST EXPERIMENTAL SHIGELLOSIS WITH A LIVING ATTENUATED ORAL POLYVALENT DYSENTERY VACCINE
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DOI：
10.1128/jb.92.1.17-22.1966
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
LABREC, EH
中科院分区：
文献类型：
--
作者：
FORMAL, SB;KENT, TH;LABREC, EH
将福氏志贺氏菌Lb、福氏志贺氏菌3和宋内氏志贺氏菌分别与大肠杆菌K-12的HFR菌株交配，以杂交菌为木糖标记。每种血清型各选一株杂交菌株进行生物学特性研究。它们对饥饿的豚鼠造成致命性肠道感染的能力降低，给猴子喂食时没有引起痢疾，它们引起豚鼠眼睛的角结膜炎，并穿透HeLa细胞。用福氏1b、2a、3型福氏志贺氏菌和宋内氏志贺氏菌I杂交株组成的多价口服疫苗，每隔4~7天给猴群灌服两剂，最后一剂免疫后10天，与一株或另一株强毒的亲本痢疾株一起攻击。除一组受到福氏志贺氏菌6挑战外，所有接种组都提供了显著程度的保护，这是疫苗中未包括的一种成分。当动物在口服疫苗后1个月被强毒福氏2a攻击时，它们也获得了保护。疫苗产生了血清抗体的增加，但我们无法在接种疫苗的动物的粪便盐水提取物中检测到辅助抗体。
Virulent strains of Shigella flexneri lb, S. flexneri 3, and S. sonnei I were mated with an Hfr strain of Escherichia coli K-12, and hybrids were selected for the xylose marker. One hybrid strain of each of the serotypes was chosen for study of their biological characteristics. Their capacity to cause a fatal enteric infection in starved guinea pigs was reduced, they failed to cause dysentery when fed to monkeys, they caused keratoconjunctivitis in the guinea pig eye, and they penetrated HeLa cells. Two doses of a polyvalent oral vaccine composed of S. flexneri lb, 2a, and 3, and S. sonnei I hybrid strains were fed to groups of monkeys at an interval of 4 to 7 days, and they, together with controls, were challenged 10 days after the last dose with one or another of the virulent parent dysentery strains. A significant degree of protection was afforded in all vaccinated groups with the exception of one group challenged with S. flexneri 6, a component not included in the vaccine. When animals were challenged with virulent S. flexneri 2a 1 month after oral vaccination, they were also protected. The vaccine produced a rise in serum antibody but we were not able to detect coproantibody in saline extracts of feces from animals which received the vaccine.