Morphological changes (including filamentation) in Escherichia coli grown under starvation conditions on silicon wafers and other surfaces
Morphological changes (including filamentation) in Escherichia coli grown under starvation conditions on silicon wafers and other surfaces
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DOI：
10.1046/j.1365-2672.1999.00602.x
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发表时间：
1999-01-01
影响因子：
2.4
通讯作者：
Reeves, CL
中科院分区：
文献类型：
--
作者：
Wainwright, M;Canham, LT;Reeves, CL
使用扫描电子显微镜，多形性（显着recreneration）时，大肠杆菌生长在饥饿条件下为14天的微孔硅晶片，钛，玻璃和塑料盘。在这些条件下，“标准”的杆状细胞（1-3 μ m）在分裂后不能分离，并产生细丝，有些长达50 μ m，许多显示出球根状尖端。在饥饿条件下，5 d后开始出现丝状体形成。还观察到哑铃形细胞，尽管明显的“Y”和“V”形细胞被证明是由重叠杆引起的伪影。本文还讨论了E.大肠杆菌，当生长在饥饿条件下，讨论了其致病性和在环境中的生长。
Using a scanning electron microscope, pleomorphism (notably filamentation) was seen when Escherichia coli was grown under starvation conditions for 14 d on microporous silicon wafers, titanium, glass and plastic discs. Under these conditions, the 'standard', rod shaped cell (1-3 mu m) failed to separate after division and filaments developed, some as long as 50 mu m, with many showing bulbous tips. Filamentation began to occur 5 d after the imposition of starvation conditions. Dumbbell shaped cells were also observed, although apparent 'Y' and 'V'-shaped cells proved to be artefacts, caused by overlapping rods. The implications of the appearance of pleomorphism in E. coli, when grown under starvation conditions, is discussed in relation to its pathogenicity and growth in the environment.