Dynamic viscoelastic properties of collagen gels with high mechanical strength
Dynamic viscoelastic properties of collagen gels with high mechanical strength
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DOI：
10.1016/j.msec.2013.03.047
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发表时间：
2013-08-01
影响因子：
7.9
通讯作者：
Hara, Masayuki
中科院分区：
文献类型：
--
作者：
Mori, Hideki;Shimizu, Kousuke;Hara, Masayuki
我们开发了一种新的方法，用于制备机械强度强的胶原蛋白凝胶相结合的连续基本凝胶形成，随后1-乙基-3-（3-二甲氨基丙基）碳二亚胺（EDC）交联和冻干。用这种方法交联三次的凝胶显示出更强的力学性能（G ′：3730 +/-2060 Pa，G ″：288 +/-35 Pa）比用EDC顺序交联一次的常规凝胶更好（G '：226 +/-70 Pa，G“：21 +/-4.4 Pa），但不如在80 ℃加热30 min的相同凝胶那么强（G '：7010 +/- 830 Pa，G“：288 +/- 35 Pa）。将常规胶原凝胶与EDC交联一次，加热一次，然后进行两次冻干-凝胶形成-交联循环，得到三循环凝胶2。该凝胶具有测试凝胶中最强的机械性能（G '：40，200 +/-18，000 Pa，G''：3090 +/- 1400 Pa，杨氏模量：0.197 +/- 0.069 MPa）。这些有前途的结果表明，凝胶作为支架在组织工程研究的可能应用。(c)2013 Elsevier B. V.保留所有权利。
We developed a new method for the preparation of mechanically strong collagen gels by combining successively basic gel formation, followed by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) cross-linking and lyophilization. Gels cross-linked three times with this method showed stronger mechanical properties (G': 3730 +/- 2060 Pa, G '': 288 +/- 35 Pa) than a conventional gel that was sequentially cross-linked with EDC once (G': 226 +/- 70 Pa, G '': 21 +/- 4.4 Pa), but not as strong as the same gel with heating for 30 min at 80 degrees C (G': 7010 +/- 830 Pa, G '': 288 +/- 35 Pa) reported in our previous paper. The conventional collagen gel was cross-linked with EDC once, heated once, and then subjected twice to a lyophilization-gel formation-cross-linking cycle to give three-cycled gel 2. This gel had the strongest mechanical properties (G': 40,200 +/- 18,000 Pa, G '': 3090 +/- 1400 Pa, Young's modulus: 0.197 +/- 0.069 MPa) of the gels tested. These promising results suggest possible applications of the gels as scaffolds in tissue engineering research. (c) 2013 Elsevier B.V. All rights reserved.