Tracing of cells of the avian thymus through embryonic life in interspecific chimeras
Tracing of cells of the avian thymus through embryonic life in interspecific chimeras
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通过种间嵌合体的胚胎生命追踪禽类胸腺细胞
DOI：
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发表时间：
1975
影响因子：
15.3
通讯作者：
F. Jotereau
中科院分区：
文献类型：
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作者：
N. L. Le Douarin;F. Jotereau
两种鸟类，日本鹌鹑（Coturnix coturnix japonica）和鸡（Gallus gallus）的间期核结构的差异已被用来区分不同来源的细胞在种间组合。将这种生物细胞标记技术应用于胸腺组织发生。使用鹌鹑和鸡胸腺雏形的成分之间的各种组合，内胚层上皮细胞，间充质细胞，和血液传播的外源性元素胸腺的组织发生的各自的贡献进行了分析。结果表明，胸腺的整个淋巴细胞群是来自移民的血源性干细胞，这些干细胞被第三和第四咽囊的内胚层化学吸引。后者决定在15-体节阶段就分化成胸腺上皮网，并且即使在异位位置（例如胚胎的腹体壁）移植时也能够吸引造血干细胞。结果表明，胸腺间充质起源于神经嵴中外胚层，并在第三和第四鳃弓早期定植。它参与血管周围间充质的形成，但不产生淋巴细胞。从异源移植鹌鹑胸腺到鸡胚（反之亦然）在不同的发展阶段出现的胸腺雏形成为淋巴干细胞的吸引力，在其进化的精确阶段，为每个物种。对鹌鹑的引诱期约为24 h，对雏鸡的引诱期约为36 h。然后，干细胞的流入变得非常低，直到孵育期结束。此时，第二波淋巴母细胞侵入胸腺，原始胚胎淋巴细胞群在孵化时完全更新。有能力的胸腺干细胞存在于生理胸腺吸引期前后的血液中。鹌鹑-雏鸡嵌合胸腺的分析证实了胸腺组织发生过程中出现的嗜碱性细胞和淋巴干细胞的同一性。
Differences in the structure of the interphase nucleus between two species of birds, the Japanese quail (Coturnix coturnix japonica) and the chick (Gallus gallus) has been used to distinguish cells from different origins in interspecies combinations. This biological cell marking technique was applied to thymus histogenesis. Using various combinations between components of quail and chick thymic rudiments, the respective contribution of endodermal epithelium, mesenchyme, and blood-borne extrinsic elements to the histogenesis of thymus was analyzed. It was demonstrated that the whole lymphoid population of the thymus is derived from immigrant blood-borne stem cells which are chemically attracted by the endoderm of the 3rd and 4th pharyngeal pouch. The latter is determined to differentiate into thymic epithelial reticulum as soon as the 15-somite stage, and is able to attract blood stem cells even when transplanted in an heterotopic position such as the ventral body wall of the embryo. It was shown that the thymic mesenchyme originates from the neural crest mesectoderm which colonizes early the 3rd and 4th branchial arches. It participates in the formation of perivascular mesenchyme, but does not give rise to lymphocytes. From heterospecific transplantations of quail thymuses into chick embryo (and inversely) at various stages of development is appeared that the thymic rudiment becomes attractive for lymphoid stem cells at a precise stage of its evolution for each species. The attractivity period lasts about 24 h for the quail and 36 h for the chick. Then, the inflow of stem cells becomes very low until the end of the incubation period. At this time, a second wave of lymphocytoblasts invades the thymus and the primitive embryonic lymphoid population is completely renewed around the hatching time. Competent thymic stem cells are present in the blood before and after the period of physiological thymic attractivity. The identity of basophilic cells appearing in the thymus during its histogenesis and lymphoid stem cells has been demonstrated from the analysis of quail-chick chimeric thymuses.