Prunus microsatellite marker transferability across rosaceous crops
Prunus microsatellite marker transferability across rosaceous crops
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DOI：
10.1007/s11295-010-0284-z
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发表时间：
2010-10-01
影响因子：
2.4
通讯作者：
Arus, Pere
中科院分区：
文献类型：
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作者：
Mnejja, Mourad;Garcia-Mas, Jordi;Arus, Pere
利用145对微卫星引物对李属9个蔷薇科植物的8个品种进行了遗传转移性研究（杏仁、桃、杏、日本李、欧洲李、樱桃、苹果、梨和草莓），杏仁基因组、桃基因组、桃表达序列标签（EST）和日本李基因组各25个，杏仁EST，杏23个（EST 13个，基因组10个），均为单位点多态性重复序列。大多数引物（83.6%）在其它李属中扩增出预期大小的条带。可转移性，即，在李属中扩增出多态性的微卫星比例也较高（63.9%）。杏仁和日本李是最易变的二倍体物种（所有，但六倍体欧洲李）和桃的多态性最少。在所有研究的李属物种中，有31个微卫星被扩增并具有多态性，其中12个微卫星覆盖了其整个基因组，被认为是“通用李属集”。相反，只有16.3%是可转移的蔷薇科其他属（苹果，梨，草莓）的物种。多态性李微卫星也检测到较低水平的变异性在非同源物种。EST和基因组来源的微卫星之间的可转移性和检测变异的能力没有显着差异。
A total of 145 microsatellite primer pairs from Prunus DNA sequences were studied for transferability in a set of eight cultivars from nine rosaceous species (almond, peach, apricot, Japanese plum, European plum, cherry, apple, pear, and strawberry), 25 each of almond genomic, peach genomic, peach expressed sequence tags (EST), and Japanese plum genomic, 22 of almond EST, and 23 of apricot (13 EST and 10 genomic), all known to produce single-locus and polymorphic simple-sequence repeats in the species where they were developed. Most primer pairs (83.6%) amplified bands of the expected size range in other Prunus. Transferability, i.e., the proportion of microsatellites that amplified and were polymorphic, was also high in Prunus (63.9%). Almond and Japanese plum were the most variable among the diploid species (all but the hexaploid European plum) and peach the least polymorphic. Thirty-one microsatellites amplified and were polymorphic in all Prunus species studied, 12 of which, covering its whole genome, are proposed as the "universal Prunus set". In contrast, only 16.3% were transferable in species of other Rosaceae genera (apple, pear, and strawberry). Polymorphic Prunus microsatellites also detected lower levels of variability in the non-congeneric species. No significant differences were detected in transferability and the ability to detect variability between microsatellites of EST and genomic origin.