Adenosine A1 receptors modulate anxiety in CD1 mice
Adenosine A1 receptors modulate anxiety in CD1 mice
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DOI：
10.1007/s002130050572
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发表时间：
1998-04-01
影响因子：
3.4
通讯作者：
Vertua, R
中科院分区：
文献类型：
--
作者：
Florio, C;Prezioso, A;Vertua, R
用高架正迷宫和明暗试验两种测定啮齿动物焦虑的模型，观察了选择性腺苷Al受体激动剂2-氯-N-6-环戊基腺苷(CCPA)对CD1小鼠的影响。在高架正迷宫实验中，CCPA在0.3nmol/kg剂量下具有抗焦虑作用；在明暗实验中，CCPA在1nmol/kg剂量下有明显的抗焦虑作用。通过体外结合实验，发现给予100nmol/kg CCPA后，脑内22 nM的水平。这些值与CCPA对腺苷A(1)受体的占有率一致，但与A(2)受体的占有率无关，提示CCPA的抗焦虑作用可能是由位于中央位置的腺苷A(1)受体介导的。在两个试验中，选择性腺苷A(1)受体拮抗剂CPT和非选择性腺苷拮抗剂IBMX都有引起焦虑的作用。因此，嘌呤能神经元可能参与了小鼠情感状态的紧张性调节。
The effect of the selective adenosine Al receptor agonist 2-chloro-N-6-cyclopentyladenosine (CCPA) was investigated in CD1 mice by the elevated plus-maze and the light/dark test, two models for measuring anxiety in rodents. CCPA, administered IP, had an anxiolytic effect at 0.3 nmol/kg in the elevated plus-maze and at 1 nmol/kg in the light/dark test. Brain levels of 22 nM were found after administration of 100 nmol/kg CCPA, as measured by ex vivo binding experiments. These values are consistent with the occupancy of adenosine A(1) but not A(2) receptors by CCPA, and suggest that the anxiolytic-like action of CCPA may be mediated by centrally located adenosine A(1) receptors. Both CPT, a selective adenosine A(1) receptor antagonist, and IBMX, a non-selective adenosine antagonist, had an anxiogenic effect in the two tests. It is thus possible that purinergic neurons may be involved in the tonic modulation of affective state in mice.