Evaluation of antibiotic combination of Litsea cubeba essential oil on Vibrio parahaemolyticus inhibition mechanism and anti-biofilm ability
Evaluation of antibiotic combination of Litsea cubeba essential oil on Vibrio parahaemolyticus inhibition mechanism and anti-biofilm ability
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DOI：
10.1016/j.micpath.2022.105574
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发表时间：
2022-05-20
影响因子：
3.8
通讯作者：
Xue, Zhenglian
中科院分区：
文献类型：
--
作者：
Li, Anqi;Shi, Chenglong;Xue, Zhenglian
副溶血性弧菌（Vibrio parahaemolyticus）是海产品中常见的致病菌。抗生素的使用是水产养殖业预防和控制副溶血性弧菌的主要手段。然而，副溶血性弧菌通过在某些条件下形成生物膜来对抗抗生素引起的损害。通过测定山苍子精油（LCEO）与几种常用的副溶血性弧菌抗生素组合的最小抑菌浓度（MIC）和部分抑菌浓度指数（FICI），分析了山苍子精油对副溶血性弧菌的抗菌作用及其特性。用时间-杀菌曲线评价精油单独和精油与抗生素组合的杀菌效果。以副溶血性弧菌ATCC 17802为实验菌株，通过测定培养上清液中大分子物质和碱性磷酸酶（AKP）的含量来评价细胞膜和细胞壁的损伤程度。透射电镜观察细胞膜结构。结果表明，LCEO的MIC值为1，024 μ g/mL，与四环素或盐酸土霉素组合的LCEO FICI值分别为0.3125和0.75，表明协同和相加效应。此外，LCEO通过降低细胞表面疏水性多糖和胞外多糖的含量，抑制了细胞的生长，促进了生物膜的去除。本研究为研究LCEO的抗菌活性及联合抗生素防治副溶血性弧菌生物膜的形成提供了参考。
Vibrio parahaemolyticus (V. parahaemolyticus) is a common pathogen in seafood. The use of antibiotics is a pri-mary tool to prevent and control V. parahaemolyticus in the aquaculture industry. However, V. parahaemolyticus combats the damage caused by antibiotics by forming biofilms under certain conditions. In this study, we analyzed the antibacterial effect and the characteristics of V. parahaemolyticus by experimentally determining the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI) values of a combination of the Litsea cubeba essential oil (LCEO) and several commonly used V. parahaemolyticus antibiotics. The bactericidal effect of the essential oil alone and essential oil in combination with the antibiotics were evaluated with time-kill curves. The damage to cell membranes and cell walls were assessed by measuring the content of macromolecules and alkaline phosphatase (AKP) released into the supernatant using V. parahaemolyticus ATCC17802 as the experimental strain. The membrane structure was observed by trans-mission electron microscopy. The results showed that the MIC value of the LCEO was 1,024 mu g/mL, and the LCEO FICI values in combination with tetracycline or oxytetracycline hydrochloride was 0.3125 and 0.75, respectively, indicating synergistic and additive effects. Moreover, LCEO inhibited the growth and promoted the removal of biofilms by reducing the content of hydrophobic and extracellular polysaccharides on the cell surface. This study provides a reference for studying the antibacterial activity of LCEO and the combination of antibiotics to prevent and control the formation of biofilms by V. parahaemolyticus.