Cytokine expression and molecular mechanisms of their auto/paracrine regulation of anterior pituitary function and growth
Cytokine expression and molecular mechanisms of their auto/paracrine regulation of anterior pituitary function and growth
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DOI：
10.1111/j.1749-6632.1998.tb09591.x
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发表时间：
1998-01-01
影响因子：
2.4
通讯作者：
Stalla, GK
中科院分区：
文献类型：
--
作者：
Arzt, E;Pereda, MP;Stalla, GK
脑下垂体表达细胞因子及其受体。IL-2受体转录本和蛋白产物在ACTH、PRL和GH产生细胞中共定位(双重免疫荧光)。IL-2和IL-6(1-1000IU/ml)参与正常和肿瘤(GH(3)乳腺癌细胞株和腺瘤细胞培养)的自分泌/旁分泌调节，包括垂体前叶激素产生细胞的生长(细胞数量、DNA合成、c-fos基因表达和放射自显影结合激素染色)。IL-1调节正常垂体细胞的生长，但不作用于GH，细胞。阻断这一作用的IL-1ra在肿瘤垂体(主要是GH-和ACTH-)细胞中表达。在ACTH细胞中，IL-1增强糖皮质激素反馈，刺激糖皮质激素反应元件转录活性。细胞因子通过特定的功能受体发挥细胞间/自体因子的作用，不仅调节垂体前叶细胞的功能，而且调节其生长。
The pituitary gland expresses cytokines and their receptors. IL-2 receptor transcripts and protein products are co-localized in ACTH-, PRL-, and GH-producing cells (double immunofluorescence). IL-2 and IL-6 (1-1000 IU/ml) are involved in the autocrine/paracrine regulation of normal and tumor (GH(3) mammosomatotroph cell line and adenoma cell cultures) anterior pituitary hormone-producing cell growth (cell number, DNA synthesis, c-fos mRNA expression and autoradiography combined with hormone staining). IL-1 regulates the growth of normal pituitary cells but does not act on GH, cells. IL-1ra, which blocks this action, is expressed in tumoral pituitary (mainly GH- and ACTH-) cells. In ACTH- cells, IL-1 enhances glucocorticoid feedback, stimulating glucocorticoid response element transcriptional activity. Cytokines, through specific functional receptors, act as inter/auto-cellular factors that regulate not only the function but also the growth of anterior pituitary cells.