Expression of placental leucine aminopeptidase is associated with a poor outcome in endometrial endometrioid adenocarcinoma
Expression of placental leucine aminopeptidase is associated with a poor outcome in endometrial endometrioid adenocarcinoma
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DOI：
10.1159/000078329
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发表时间：
2004-01-01
期刊：
影响因子：
3.5
通讯作者：
Mizutani, S
中科院分区：
文献类型：
--
作者：
Shibata, K;Kikkawa, F;Mizutani, S
目的：胎盘亮氨酸氨基肽酶(P-LAP)是一种细胞表面氨基肽酶(催产素酶)。我们克隆了PLAP基因，发现P-LAP在组织中广泛分布。由于P-LAP可以降解催产素等几种小肽激素，这种酶可能会影响癌细胞和正常细胞的许多细胞功能。本研究探讨P-LAP在子宫内膜样腺癌中的表达与临床病理因素及预后的关系。方法：采用多克隆P-LAP抗体对99例子宫内膜癌石蜡包埋组织切片进行P-LAP染色。根据P-LAP染色强度分析无瘤生存期和其他临床病理特征。结果：99例中69例(69.7%)P-LAP免疫组化染色阳性。我们发现P-LAP的表达与组织学分级(p<；0.01)、手术分期(p=0.02)、子宫肌层侵犯(p=0.01)、淋巴结转移(p<；0.01)和血管浸润性(p<0.05)呈正相关。
Objective: Placental leucine aminopeptidase (P-LAP) is a cell surface aminopeptidase (oxytocinase). We cloned PLAP cDNA and found a widespread tissue distribution of P-LAP. Since P-LAP can degrade several small peptide hormones such as oxytocin, this enzyme may affect many cellular functions of carcinoma cells as well as normal cells. This study investigated whether the expression of P-LAP correlates with clinicopathologic factors and prognosis in patients with endometrial endometrioid adenocarcinoma. Methods: Histologic sections of formalin-fixed, paraffin-embedded specimens from 99 primary endometrial carcinomas were stained for P-LAP using polyclonal P-LAP antibody. Disease-free survival and other clinicopathologic characteristics were analyzed according to the intensity of P-LAP staining. Results: Of 99 cases, 69 (69.7%) showed specific P-LAP immunostaining. We found a positive correlation between the expression of P-LAP and histological grade (p < 0.01), surgical stage of the disease ( p = 0.02), myometrial invasion ( p = 0.01), lymph node involvement ( p < 0.01), and vascular infiltration ( p