MiR146a and MiR499 gene polymorphisms in Iranian periodontitis and peri-implantitis patients.
MiR146a and MiR499 gene polymorphisms in Iranian periodontitis and peri-implantitis patients.
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DOI：
10.1615/jlongtermeffmedimplants.2013007073
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发表时间：
2013
影响因子：
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通讯作者：
M. Kadkhodazadeh;A. Jafari;R. Amid;A. Ebadian;M. Alipour;Fatemeh Mollaverdi;A. Lafzi
中科院分区：
文献类型：
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作者：
M. Kadkhodazadeh;A. Jafari;R. Amid;A. Ebadian;M. Alipour;Fatemeh Mollaverdi;A. Lafzi
MicroRNAs （MiRs）是一种小的非编码rna，参与蛋白质翻译、破骨细胞发生和免疫调节。种植周炎和慢性牙周炎是多因素疾病。它们是宿主反应、遗传和环境复杂相互作用的结果。在本研究中，我们旨在研究MiR146a/MiR499基因多态性与牙周炎/种植周炎之间的可能关联，这是口腔种植学的首次报道。方法选取在Shahid Beheshti牙科学校牙周病科就诊的197例患者，分为慢性牙周炎（CP）组75例，种植周炎（PI）组38例，健康对照84例。从参与者手臂静脉的新鲜血液样本中提取DNA，并转移到KBiosience研究所（Hoddesdon, uk）进行基因分型。采用SPSS v.19软件进行χ 2检验和Kruskal-Wallis检验。p值< 0.05被认为是显著的。结果三组间MiR 146a和MiR 499基因型频率差异有统计学意义。结论：MiR146a （rs2910146）和MiR499 （rs3746444）基因多态性可能是伊朗人慢性牙周炎和种植周炎风险增加的遗传决定因素。为了确定这些snp的影响，需要在不同的人群中进行更多的研究，样本量更大。
AIM MicroRNAs (MiRs) are small noncoding RNAs that are involved in protein translation, osteoclastogenesis, and immunoregulation. Peri-implantitis and chronic periodontitis are multifactorial diseases. They are the consequence of complex interactions among host response, genetics, and environment. In the present study, we aimed to investigate the possible association between MiR146a/MiR499 gene polymorphisms and periodontitis/peri-implantitis as a first report in oral implantology. METHODS From 197 individuals referred to Periodontology Department of Shahid Beheshti Dental School, three groups were enrolled in the assessment: 75 patients in the chronic periodontitis (CP) group, 38 patients in the peri-implantitis (PI) group, and 84 healthy subjects. DNA was extracted from fresh blood samples from the arm veins of participants and transferred to KBiosience Institute (Hoddesdon, United Kingdom) for genotyping. Chi-squared and Kruskal-Wallis tests were performed using SPSS software v.19 for statistical analyses. P-value < 0.05 was considered significant. RESULTS The genotype frequencies in MiR 146a and MiR 499 were significantly different among the three groups. CONCLUSIONS MiR146a (rs2910146) and MiR499 (rs3746444) gene polymorphisms may be genetic determinants for increased risk of chronic periodontitis and peri-implantitis in Iranians. More studies with larger sample sizes in different populations are necessary for determining the effect of these SNPs.