A transgenic self-incompatible Arabidopsis thaliana model for evolutionary and mechanistic studies of crucifer self-incompatibility.
A transgenic self-incompatible Arabidopsis thaliana model for evolutionary and mechanistic studies of crucifer self-incompatibility.
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DOI：
10.1093/jxb/erp393
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发表时间：
2010-04
影响因子：
6.9
通讯作者：
Anne C. Rea;Pei Liu;J. Nasrallah
中科院分区：
文献类型：
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作者：
Anne C. Rea;Pei Liu;J. Nasrallah
自交不亲和（SI）的分子遗传学研究在非模式自交不亲和物种中很难进行。最近，研究者建立了一个拟南芥转基因模型，用于分析十字花科植物的SI系统，该系统通过编码s -位点受体激酶（SRK）及其配体s -位点富半胱氨酸（SCR）蛋白的基因在种间转移而起作用，这两个基因分别是柱头和花粉中SI特异性的决定因素。本文综述了拟南芥的诸多优势以及该模式物种所拥有的广泛工具和资源，使得利用转基因自交不亲和的SRK-SCR植物来解决与十字花科植物SI机制和进化相关的长期问题。它还提出了一种候选基因方法的意想不到的结果，该方法旨在确定是否与先前报道的参与芸苔属SI反应的基因相关的基因和抗病所需的基因（与SI反应有许多相似之处）是拟南芥SI所必需的。这些不同的研究为以下方面提供了新的见解：SRK-SCR相互作用的特异性基础，最终抑制“自”花粉的信号级联的本质，以及与十字花科植物交配的远交和近交模式之间转换相关的生理和形态变化。
Molecular genetic studies of self-incompatibility (SI) can be difficult to perform in non-model self-incompatible species. Recently, an Arabidopsis thaliana transgenic model was developed for analysis of the SI system that operates in the Brassicaceae by inter-species transfer of genes encoding the S-locus receptor kinase (SRK) and its ligand, the S-locus cysteine-rich (SCR) protein, which are the determinants of SI specificity in the stigma and pollen, respectively. This article reviews the various ways in which the many advantages of A. thaliana and the extensive tools and resources available in this model species have allowed the use of transgenic self-incompatible SRK-SCR plants to address long-standing issues related to the mechanism and evolution of SI in the Brassicaceae. It also presents the unexpected results of a candidate gene approach aimed at determining if genes related to genes previously reported to be involved in the SI response of Brassica and genes required for disease resistance, which exhibits many similarities to the SI response, are required for SI in A. thaliana. These various studies have provided a novel insight into the basis of specificity in the SRK-SCR interaction, the nature of the signalling cascade that culminates in the inhibition of 'self' pollen, and the physiological and morphological changes that are associated with transitions between the outbreeding and inbreeding modes of mating in the Brassicaceae.