Ultrastructural changes in the midgut epithelium of unfed lone star ticks with increasing age.
Ultrastructural changes in the midgut epithelium of unfed lone star ticks with increasing age.
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DOI：
10.1093/aesa/78.1.62
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发表时间：
1985
影响因子：
2.3
通讯作者：
John P. Williams;Deborah M. Barker;J. Sauer;J. Hair;C. Ownby;H. Koch
中科院分区：
文献类型：
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作者：
John P. Williams;Deborah M. Barker;J. Sauer;J. Hair;C. Ownby;H. Koch
对美洲钝星星蜱（Amblyomma americanum（L.）随着蜱虫年龄的增加，每隔2个月检查一次田间饲养的蜱虫。还检查了28月龄的其他蜱虫。用光学显微镜观察到的形态学变化主要与肠盲囊的颜色和扩张以及马洛里三色和普鲁士蓝染色的组织染色亲和力的变化相关。超微结构上，观察到从新蜕皮到8个月大的孤星星蜱的上皮细胞中脂质、铁粒体和髓铁粒体的量的变化。检查28个月大的蜱，发现细胞内脂质很少，但溶酶体活性明显增加。在10个月、12个月和28个月大的蜱中，质膜和基底层（基底膜的一部分）的基底内折叠是常见的。这些数据表明，一些未确定比例的血餐转化为脂质，这可能是后来代谢的能量。这与先前在实验室饲养的蜱肠道细胞研究中观察到的重要差异。季节在蜱被放置在外地的影响不大，随着蜱年龄的增加肠道细胞的明显变化。
The gross morphology of the gut epithelium of adult lone star ticks ( Amblyomma americanum ) (L.) reared in the field was examined at 2-month intervals as ticks increased in age for 1 year. Additional ticks 28 months of age were also examined. Morphological changes observed with the light microscope were primarily associated with the color and distention of the gut caeca and changes in the tissue staining affinities for Mallory's trichrome and Prussian Blue stains. Ultrastructurally, changes from newly molted to 8-month-old lone star ticks were observed in the amounts of lipid, siderosomes, and myeli-nosiderosomes in cells of the epithelium. Examination of 28-month-old ticks revealed little intracellular lipid but an apparent increase in lysosomal activity. Basal infoldings of the plasma membrane and basal lamina (part of the basement membrane) were common in 10-, 12-, and 28-month-old ticks. The data suggest that some undetermined proportion of the blood meal is converted to lipid, which may be later metabolized for energy. This is an important difference from that observed in a previous study of gut cells of ticks reared in the laboratory. Season at which ticks were placed in the field had little effect on apparent changes in gut cells with increasing tick age.