Physiology and pharmacology of acetylcholinergic responses of interneurons in the antennal lobes of the moth Manduca sexta.
Physiology and pharmacology of acetylcholinergic responses of interneurons in the antennal lobes of the moth Manduca sexta.
复制标题
蛾天蛾触角叶中间神经元乙酰胆碱能反应的生理学和药理学。
DOI：
10.1007/bf00610437
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hildebrand,JG
中科院分区：
文献类型：
--
作者：
Waldrop,B;Hildebrand,JG
1.天蛾触角叶（AL）的神经元对乙酰胆碱（ACh）的压力注射产生反应。当突触传递未被阻断时，既可以看到兴奋性反应（图2），也可以看到抑制性反应（图3）。2.对ACh的反应似乎是受体介导的，因为它们与输入电导的增加有关（图1A和1B）。图2B和3B）并且是剂量依赖性的（图2C）。3.所有对乙酰胆碱有反应的神经元也会被尼古丁兴奋。对尼古丁的反应比对乙酰胆碱的反应更强和更长（图4C）。未观察到对毒蕈碱激动剂氧震颤素的反应（图4 B）。4.箭毒阻断AL神经元对所施加的ACh的反应，而阿托品和右乙替米特在减少ACh反应方面仅具有微弱的效果（图1A和1B）。第5和6段）。5.箭毒在减少AL神经元的突触介导的反应方面也比阿托品或右乙替米胺更有效（图7）。6.在一个AL神经元中，甲碘荷包牡丹碱（BMI）阻断了电刺激触角神经产生的IPSP，但它没有减少对ACh的抑制反应（图8）。
1. Neurons in the antennal lobe (AL) of the moth Manduca sexta respond to the application, via pressure injection into the neuropil, of acetylcholine (ACh). When synaptic transmission is not blocked, both excitatory (Fig. 2) and inhibitory (Fig. 3) responses are seen. 2. Responses to ACh appear to be receptor-mediated, as they are associated with an increase in input conductance (Figs. 2B and 3B) and are dose-dependent (Fig. 2 C). 3. All neurons responsive to ACh are also excited by nicotine. Responses to nicotine are stronger and more prolonged than responses to ACh (Fig. 4C). No responses are observed to the muscarinic agonist, oxotremorine (Fig. 4 B). 4. Curare blocks responses of AL neurons to applied ACh, while atropine and dexetimide are only weakly effective at reducing ACh responses (Figs. 5 and 6). 5. Curare is also more effective than atropine or dexetimide at reducing synaptically-mediated responses of AL neurons (Fig. 7). 6. In one AL neuron, bicuculline methiodide (BMI) blocked the IPSP produced by electrical stimulation of the antennal nerve, but it did not reduce the inhibitory response to application of ACh (Fig. 8).