Differential gene expression between chronic hepatitis B and C hepatic lesion
Differential gene expression between chronic hepatitis B and C hepatic lesion
复制标题
DOI：
10.1053/gast.2001.22468
复制
发表时间：
2001-03-01
期刊：
影响因子：
29.4
通讯作者：
Kobayashi, K
中科院分区：
文献类型：
--
作者：
Honda, M;Kaneko, S;Kobayashi, K
背景和目标：互补DNA（cDNA）微阵列技术可以同时分析数百到数千个基因的表达，我们应用cDNA微阵列技术来阐明慢性病毒性肝炎组织病变中的基因表达谱。我们制作了由1080个人cDNA组成的cDNA微阵列，并使用从6个正常人、12个慢性B型肝炎患者、12个慢性乙型肝炎患者和12个慢性乙型肝炎患者中制备的标记cDNA分析了基因表达。14例慢性丙型肝炎肝组织中，用Cy5标记的cDNA与Cy3标记的正常肝组织cDNA进行杂交，得到各基因的相对表达率。对26例患者的基因表达谱进行系统聚类分析，结果显示，根据基因表达谱的相似性，差异表达基因、B和C型肝炎病毒感染是决定聚集的重要因素，而年龄、性别和肝炎组织学则无显著性差异（P> 0.05）。在B型肝炎组织病变中，炎症相关基因占优势，而在C型肝炎组织病变中，炎症反应相关基因的表达相对占优势。结论：这些发现揭示了B型肝炎病毒和丙型肝炎病毒感染引起的肝炎发病机制中可能存在的差异分子机制，肝细胞癌经常由此发展。
Background & Aims: Complementary DNA (cDNA) microarray technology allows simultaneous expression analysis of hundreds to thousands of genes, We applied the cDNA microarray technique to clarify gene expression profiles in chronic viral hepatitis tissue lesions, Methods: We made cDNA microarrays consisting of 1080 human cDNAs and analyzed gene expression using labeled cDNAs prepared from 6 normal, 12 chronic hepatitis B, and 14 chronic hepatitis C liver tissues, Relative expression ratios of individual genes were obtained by comparing hybridization of Cy5-labeled cDNAs from chronic hepatitis lesions and Cy3-labeled cDNA from normal liver tissue, Results: Hierarchical clustering analysis of the gene expression profiles in 26 patients showed that the patients were clustered into 2 groups with respect to similarities in differentially expressed genes, Hepatitis B and C virus infection, but not age, sex, or histology of hepatitis, were significant factors determining clustering (P < 0.05), In hepatitis B tissue lesions, genes involved in inflammation were predominant, whereas in hepatitis C, expression of antiinflammatory response genes was relatively dominant. Conclusions: These findings shed new light on the possible differential molecular mechanisms in the pathogenesis of hepatitis caused by hepatitis B virus and hepatitis C virus infection, from which hepatocellular carcinoma frequently develops.