Association of the HGF/SF receptor, c-met, with the cell-surface adhesion molecule, E-cadherin, and catenins in human tumor cells
Association of the HGF/SF receptor, c-met, with the cell-surface adhesion molecule, E-cadherin, and catenins in human tumor cells
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DOI：
10.1006/bbrc.1999.1002
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发表时间：
1999-08-02
影响因子：
3.1
通讯作者：
Jiang, WG
中科院分区：
文献类型：
--
作者：
Hiscox, S;Jiang, WG
肿瘤细胞的转移潜力在用HGF/SF （c-met受体酪氨酸激酶的配体）治疗后显著增强。肿瘤细胞中c-met激活后，β -连环蛋白发生磷酸化，同时细胞间粘连丧失，细胞的运动性和侵袭性增强。在这项研究中，我们发现c-met在人类结肠癌（HRT18和HT115）和两种乳腺癌（MCF7和MDA MB 231）细胞系的细胞-细胞接触区域与β -连环蛋白和e -钙粘蛋白共定位。免疫沉淀研究表明，在这些上皮肿瘤细胞中，c-met与钙粘着蛋白复合体成员以及膜酪氨酸蛋白磷酸酶（PTP mu）之间存在关联。我们得出结论，HGF/SF受体C -met与cadherin/ catenin细胞-细胞粘附系统成员和PTP mu可能在E-cadherin阳性肿瘤细胞中形成蛋白质复合物的一部分，在HGF/SF刺激后调节细胞间粘附(C)， 1999年学术出版社。
Tumour cell metastatic potential is significantly enhanced following treatment with HGF/SF, the ligand for the c-met receptor tyrosine kinase, Following c-met activation in tumour cells, phosphorylation of beta-catenin occurs, together with loss of intercellular adhesion and a gain in the motile and invasive nature of the cell. In this study we show that c-met is colocalised with beta-catenin and E-cadherin at regions of cell-cell contact in human colon cancer (HRT18 and HT115) and two breast cancer (MCF7 and MDA MB 231) cell lines. Immunoprecipitation studies demonstrated an association between c-met and members of the cadherin adhesion complex in these epithelial tumour cells, along with the membrane tyrosine protein phophatase, PTP mu. We conclude that the HGF/SF receptor, c-met, together with members of the cadherin/ catenin cell-cell adhesion system and PTP mu, may form part of a protein complex in E-cadherin positive tumour cells that acts to regulate intercellular adhesion following HGF/SF stimulation, (C) 1999 Academic Press.