Genome to phenome tools: In vivo and in vitro transfection of Crassostrea virginica hemocytes
Genome to phenome tools: In vivo and in vitro transfection of Crassostrea virginica hemocytes
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基因组到表型工具：维吉尼亚牡蛎血细胞的体内和体外转染
DOI：
10.1016/j.fsi.2020.05.022
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发表时间：
2020
影响因子：
4.7
通讯作者：
Fernández Robledo, José A.
中科院分区：
文献类型：
--
作者：
Tracy, Adrienne N.;Yadavalli, Raghavendra;Reed, Kiara S.;Parnaik, Rahul;Poulton, Nicole J.;Bishop-Bailey, David;Fernández Robledo, José A.
维吉尼亚牡蛎基因组的测序重新引起了人们对基因传递和编辑方法的兴趣。在这里，我们报告了两种异源表达载体在用树枝状聚合物递送的 CMV 启动子下在牡蛎血细胞中的表达。使用共聚焦显微镜、流式细胞术和免疫荧光测定法监测表达。C.维吉尼亚血细胞在CMV病毒启动子的作用下在体内和体外均能够表达绿色荧光蛋白和巨牡蛎血管内皮生长因子。这些结果为询问基因组和调整基因组编辑方法提供了基础。
The sequencing of theCrassostrea virginicagenome has brought back the interest for gene delivery and editing methodologies. Here, we report the expression in oyster hemocytes of two heterologous expression vectors under the CMV promoter delivered with dendrimers. Expression was monitored using confocal microscopy, flow cytometry, and immunofluorescence assay.C. virginicahemocytes were able to express the green fluorescence protein andCrassostrea gigasvascular endothelial growth factor under CMV viral promoter bothin vivoandin vitro. These results provide the bases for interrogating the genome and adapting genome editing methodologies.