Identification of 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A) as a novel RNA modification at position 37 of tRNAs.
Identification of 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A) as a novel RNA modification at position 37 of tRNAs.
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DOI：
10.1093/nar/gkw1120
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发表时间：
2017-02-28
影响因子：
14.9
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Kang BI;Miyauchi K;Matuszewski M;D'Almeida GS;Rubio MAT;Alfonzo JD;Inoue K;Sakaguchi Y;Suzuki T;Sochacka E;Suzuki T
转移RNA修饰在蛋白质合成中起着至关重要的作用。N6-苏氨酰氨基甲酰腺苷(T6A)及其衍生物是在负责ANN密码子的tRNA中37，3΄-邻近反密码子的位置上发现的修饰。这些修改在生活的所有领域都是普遍保守的。T6A及其衍生物在蛋白质合成中具有多种功能，包括氨酰化、解码和转位。我们以前在细菌、真菌、植物和原生生物的tRNA中发现了一种环形式的t6A(Ct6A)，它是t6A的一种化学不稳定的衍生物。在这里，我们报道了2-甲硫基环t6A(Ms2ct6A)，它是从枯草芽孢杆菌、植物和布鲁氏锥虫的tRNA中发现的ct6A的新衍生物。在枯草芽孢杆菌和布鲁氏芽孢杆菌中，ms2ct6A分别由于TcdA和mtaB同源基因的缺失而消失，并保留为ms2t6A和ct6A，表明TcdA和mtaB参与了ms2ct6A的生物发生。
Transfer RNA modifications play pivotal roles in protein synthesis. N6-threonylcarbamoyladenosine (t6A) and its derivatives are modifications found at position 37, 3΄-adjacent to the anticodon, in tRNAs responsible for ANN codons. These modifications are universally conserved in all domains of life. t6A and its derivatives have pleiotropic functions in protein synthesis including aminoacylation, decoding and translocation. We previously discovered a cyclic form of t6A (ct6A) as a chemically labile derivative of t6A in tRNAs from bacteria, fungi, plants and protists. Here, we report 2-methylthio cyclic t6A (ms2ct6A), a novel derivative of ct6A found in tRNAs from Bacillus subtilis, plants and Trypanosoma brucei. In B. subtilis and T. brucei, ms2ct6A disappeared and remained to be ms2t6A and ct6A by depletion of tcdA and mtaB homologs, respectively, demonstrating that TcdA and MtaB are responsible for biogenesis of ms2ct6A.