A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the toll pathway
A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the toll pathway
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DOI：
10.1093/emboj/17.5.1217
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发表时间：
1998-03-02
期刊：
影响因子：
11.4
通讯作者：
Hoffmann, JA
中科院分区：
文献类型：
--
作者：
Ferrandon, D;Jung, AC;Hoffmann, JA
果蝇全身抗菌反应的标志是脂肪体合成几种抗菌肽，这些抗菌肽在脓毒性损伤时释放到血淋巴中。这些肽之一，drosomycin，主要对真菌有活性。使用drosomycin-green fluorescent protein（GFP）报告基因，我们现在表明，除了脂肪体，与外部环境直接接触的各种上皮组织，包括呼吸道、消化道和生殖道的上皮组织，可以表达抗真菌肽，表明对影响这些屏障组织的感染的局部反应。脊椎动物上皮的情况也是如此，昆虫上皮似乎不仅仅是被动的物理屏障，而且可能构成先天免疫的主动成分。我们还表明，与全身抗真菌反应相反，这种局部免疫反应不依赖于Toll途径。
A hallmark of the systemic antimicrobial response of Drosophila is the synthesis by the fat body of several antimicrobial peptides which are released into the hemolymph in response to a septic injury, One of these peptides, drosomycin, is active primarily against fungi, Using a drosomycin-green fluorescent protein (GFP) reporter gene, we now show that in addition to the fat body, a variety of epithelial tissues that are in direct contact with the external environment, including those of the respiratory, digestive and reproductive tracts, can express the antifungal peptide, suggesting a local response to infections affecting these barrier tissues, As is the case for vertebrate epithelia, insect epithelia appear to be more than passive physical barriers and are likely to constitute an active component of innate immunity, We also show that, in contrast to the systemic antifungal response, this local immune response is independent of the Toll pathway.