Isolation and characterization of four ethylene signal transduction elements in plums (Prunus salicina L.)
Isolation and characterization of four ethylene signal transduction elements in plums (Prunus salicina L.)
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李子（Prunus salicina L.）中四种乙烯信号转导元件的分离与表征
DOI：
10.1093/jxb/erm213
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发表时间：
2007-10-01
影响因子：
6.9
通讯作者：
Brown, D. C. W.
中科院分区：
文献类型：
--
作者：
El-Sharkawy, I.;Kim, W. S.;Brown, D. C. W.
李子是跃变型水果：它们的成熟与乙烯的产生和呼吸速率的激增有关。核果，包括李子，具有独特的生长发育模式，被描述为双S型模式。为了了解李树对乙烯感受的发育调控，我们研究了4个(E)在胁迫(P)下的乙烯感受(P)和(S)在胁迫(T)下的胁迫(C)在胁迫组分(EPSTCs)下的特性，包括两个ETR1样蛋白(Ps-ETR1and Ps-ERS1)、一个CTR1样蛋白和一个乙烯响应元件结合因子(ERF)。在早熟和晚熟品种的果实发育和成熟过程中，研究了它们的调控。转录水平分析表明，只有Ps-ERF1和Ps-ERS1在受精后立即积累。Ps-ETR1和Ps-CTR1的转录水平在果实发育的S3之前没有检测到增加。观察到早(‘早金’)和晚(‘史郎’)日本李品种的成熟行为存在显著差异。早期品种表现出典型的跃变型果实成熟模式，并伴随着乙烯依赖的四个转录物水平的急剧增加。然而，晚熟品种表现出抑制-跃变模式，与成熟相关的乙烯产生略有增加。晚熟品种Ps-ETR1(而不是Ps-CTR1)mRNA的积累不依赖于乙烯。PS-ERS1mRNA低水平持续表达，而Ps-ERF1仍未检测到。讨论了两个李品种在成熟期和成熟率上的差异与四种mRNAs积累模式的差异。
Plums are climacteric fruits: their ripening is associated with a burst of ethylene production and respiration rate. Stone fruits, including plum, have a distinct pattern of growth and development, described as a double sigmoid pattern. In order to understand the developmental control of ethylene perception in plum, four (e) under bar thylene (p) under bar erception and (s) under bar ignal (t) under bar ransduction (c) under bar omponents (EPSTCs) were characterized, including two ETR1-like proteins (Ps-ETR1 and Ps-ERS1), a CTR1-like protein, and an ethylene-responsive element-binding factor (ERF). Their regulation was studied throughout fruit development and ripening in early and late cultivars. Analysis of transcript levels revealed that only Ps-ERF1 and Ps-ERS1 accumulated immediately after fertilization. Increases in Ps-ETR1 and Ps-CTR1 transcript levels could not be detected before S3 of fruit development. Marked differences associated with the ripening behaviour of early ('Early Golden') and late ('Shiro') Japanese plum cultivars were observed. The early cultivar showed ripening patterns typical of climacteric fruits accompanied by sharp increases of the four transcript levels in an ethylene-dependent manner. However, the late cultivar exhibited a suppressed-climacteric pattern, with a slight increase in ethylene production related to ripening. The accumulation of the Ps-ETR1 (and not Ps-CTR1) mRNA in the late cultivar was ethylene independent. Ps-ERS1 mRNA was expressed at low, constant levels, while, Ps-ERF1 remained undetectable. The differences between the two plum cultivars in the date and rate of ripening in relation to the differences in the accumulation patterns of the four mRNAs are discussed.