Novel molecular diagnostic tools for malaria elimination: a review of options from the point of view of high-throughput and applicability in resource limited settings.
Novel molecular diagnostic tools for malaria elimination: a review of options from the point of view of high-throughput and applicability in resource limited settings.
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DOI：
10.1186/s12936-016-1158-0
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发表时间：
2016-02-16
期刊：
影响因子：
3
通讯作者：
McCarthy JS
中科院分区：
文献类型：
--
作者：
Britton S;Cheng Q;McCarthy JS
随着疟疾传播持续减少，越来越多的国家将进入消灭前和消灭阶段。为了阻断传播，控制策略的改变可能需要更准确地识别所有疟原虫寄生虫携带者，包括有症状和无症状的，使用高度敏感、高通量和快速周转时间的诊断工具，最好在当地卫生服务机构进行。目前可用的免疫层析侧向流快速诊断测试和现场显微镜不太可能在寄生虫密度小于100个寄生虫/微升时一致地检测感染，使得它们对于检测所有载体不够敏感。分子诊断平台，如PCR和LAMP，目前可在参考实验室，但在财政和周转时间的成本。本文综述了近年来发展的分子诊断工具，在其高通量的能力和潜在的性能在非参考实验室消灭疟疾的最新进展。
As malaria transmission continues to decrease, an increasing number of countries will enter pre-elimination and elimination. To interrupt transmission, changes in control strategies are likely to require more accurate identification of all carriers of Plasmodium parasites, both symptomatic and asymptomatic, using diagnostic tools that are highly sensitive, high throughput and with fast turnaround times preferably performed in local health service settings. Currently available immunochromatographic lateral flow rapid diagnostic tests and field microscopy are unlikely to consistently detect infections at parasite densities less than 100 parasites/µL making them insufficiently sensitive for detecting all carriers. Molecular diagnostic platforms, such as PCR and LAMP, are currently available in reference laboratories, but at a cost both financially and in turnaround time. This review describes the recent progress in developing molecular diagnostic tools in terms of their capacity for high throughput and potential for performance in non-reference laboratories for malaria elimination.