Chemical and Molecular Composition of the Chrysalis Reveals Common Chitin-Rich Structural Framework for Monarchs and Swallowtails
Chemical and Molecular Composition of the Chrysalis Reveals Common Chitin-Rich Structural Framework for Monarchs and Swallowtails
复制标题
DOI：
10.1016/j.jmb.2022.167456
复制
发表时间：
2022-02-05
影响因子：
5.6
通讯作者：
Cegelski, Lynette
中科院分区：
文献类型：
--
作者：
Goularte, Nicolette F.;Kallem, Till;Cegelski, Lynette
从毛毛虫到蝴蝶的蜕变是一个令人敬畏的例子，说明了在自然界复杂的系统中，特殊的化学物质是如何使非凡的功能成为可能的。蛹的外骨骼显露出来，并随着毛虫向蝴蝶形态的转变而脱落。我们采用固态核磁共振来评估的化学成分和类型的生物分子的蝶蛹从君主和燕尾蝶出现。帝王蝶和燕尾蝶蛹的成分非常相似。甲壳素是主要的多糖成分，与蛋白质和儿茶酚或儿茶酚型键一起存在于每个茧中。高几丁质含量可与含几丁质最高的昆虫外骨骼相比。蛋白质组学分析表明，几丁质酶的存在下，可能参与的蛹的合成和重塑，以及角质层蛋白，发挥作用的蛹的结构完整性。几乎相同的C-13 CPMAS NMR光谱的每个蛹和类似的结构蛋白支持存在的基本设计原则，整合甲壳素和蛋白质的合作伙伴，以阐述蛹。(c)2022爱思唯尔有限公司保留所有权利。
The metamorphosis of a caterpillar into a butterfly is an awe-inspiring example of how extraordinary functions are made possible through specific chemistry in nature's complex systems. The chrysalis exoskeleton is revealed and shed as a caterpillar transitions to butterfly form. We employed solid-state NMR to evaluate the chemical composition and types of biomolecules in the chrysalides from which Monarch and Swallowtail butterflies emerged. The chrysalis composition was remarkably similar between Monarch and Swallowtail. Chitin is the major polysaccharide component, present together with proteins and catechols or catechol-type linkages in each chrysalis. The high chitin content is comparable to the highest chitin-containing insect exoskeletons. Proteomics analyses indicated the presence of chitinases that could be involved in synthesis and remodeling of the chrysalis as well as cuticular proteins which play a role in the structural integrity of the chrysalis. The nearly identical C-13 CPMAS NMR spectra of each chrysalis and similar structural proteins supports the presence of underlying design principles integrating chitin and protein partners to elaborate the chrysalis. (c) 2022 Elsevier Ltd. All rights reserved.