Transcriptome responses to heat stress in the nucleated red blood cells of the rainbow trout (Oncorhynchus mykiss)
Transcriptome responses to heat stress in the nucleated red blood cells of the rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1152/physiolgenomics.00067.2010
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发表时间：
2010-08-01
影响因子：
4.6
通讯作者：
Currie, Suzanne
中科院分区：
文献类型：
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作者：
Lewis, Johanne M.;Hori, Tiago S.;Currie, Suzanne
刘易斯JM，霍里TS，上升ML，沃尔什PJ，柯里S。虹鳟鱼（Oncorhynchus mykiss）有核红细胞对热应激的转录组反应。Physiol Genomics 42：361-373，2010.首次发表于2010年6月15日; doi：10.1152/physiolgenomics.00067.2010.-保留在成熟状态的鱼红细胞（RBC）的细胞核，并能够很容易地收集和操纵血液在非终端实验使血液一个理想的组织，在其上研究鱼类的细胞应激反应。通过使用cGRASP 16 K鲑鱼微阵列，我们研究了在对照条件下（11摄氏度）和暴露于热应激（25摄氏度1小时，然后在11摄氏度恢复）的鱼中RBC全局基因转录的差异。重复的血液采样（通过背主动脉插管）使我们能够检查个体随时间的应激反应。在预热应激（代表单独对照）和热应激后4小时和24小时（代表早期和晚期转录调控）采集样品。当与RBC RNA衍生的靶杂交时，约3，000个微阵列特征具有高于阈值的信号，并且在研究的时间点插管对RBC mRNA表达没有可检测的影响。参与应激反应、免疫反应和细胞凋亡的基因在早期和晚期转录调节期间都表现出最高的失调。此外，与血细胞分化和发育相关的基因在24小时时间点转录上调。这项研究提供了一个更广泛的理解机制的基础上的压力反应，在鱼和发现新的基因，在一个特定的方式调节压力。此外，在热应激反应中血液中持续失调的鲑鱼转录物是暴露于这种特定应激源的非致命生物标志物的潜在候选者。
Lewis JM, Hori TS, Rise ML, Walsh PJ, Currie S. Transcriptome responses to heat stress in the nucleated red blood cells of the rainbow trout (Oncorhynchus mykiss). Physiol Genomics 42: 361-373, 2010. First published June 15, 2010; doi: 10.1152/physiolgenomics.00067.2010.-The retention of a nucleus in the mature state of fish red blood cells (RBCs) and the ability to easily collect and manipulate blood in nonterminal experiments make blood an ideal tissue on which to study the cellular stress response in fish. Through the use of the cGRASP 16K salmonid microarray, we investigated differences in RBC global gene transcription in fish held under control conditions (11 degrees C) and exposed to heat stress (1 h at 25 degrees C followed by recovery at 11 degrees C). Repeated blood sampling (via a dorsal aorta cannula) enables us to examine the individual stress response over time. Samples were taken preheat stress (representing individual control) and at 4 and 24 h postheat stress (representing early and late transcriptional regulation). Approximately 3,000 microarray features had signal above threshold when hybridized with RBC RNA-derived targets, and cannulation did not have a detectable effect on RBC mRNA expression at the investigated time points. Genes involved in the stress response, immune response, and apoptosis were among those showing the highest dysregulation during both early and late transcriptional regulation. Additionally, genes related to the differentiation and development of blood cells were transcriptionally upregulated at the 24 h time point. This study provides a broader understanding of the mechanisms underpinning the stress response in fish and the discovery of novel genes that are regulated in a stress specific manner. Moreover, salmonid transcripts that are consistently dysregulated in blood in response to heat stress are potential candidates of nonlethal biomarkers of exposure to this particular stressor.