Human CD8+ CXCR3+ T cells have the same function as murine CD8+ CD122+ Treg
Human CD8+ CXCR3+ T cells have the same function as murine CD8+ CD122+ Treg
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DOI：
10.1002/eji.200939314
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发表时间：
2009-08-01
影响因子：
5.4
通讯作者：
Suzuki, Haruhiko
中科院分区：
文献类型：
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作者：
Shi, Zhe;Okuno, Yusuke;Suzuki, Haruhiko
先前已经在小鼠中证明了CD 8（+）CD 122（+）Treg在维持免疫稳态中的重要性。由于人中CD 8和CD 122的表达模式与小鼠中不同，因此尚未鉴定出对应于鼠CD 8（+）CD 122（+）Treg的人CD 8（+）Treg。在本研究中，我们通过DNA微阵列分析比较了小鼠CD 8（+）CD 122（+）细胞和CD 8（+）CD 122（-）细胞的基因表达谱，发现CXCR 3在CD 8（+）CD 122（+）细胞中优先表达。当我们分析小鼠CD 8（+）细胞中CD 122和CXCR 3的表达时，我们观察到了确定的CD 122（+）CXCR 3（+）细胞群体。通过体内和体外试验，小鼠中的CD 8（+）CXCR 3（+）细胞显示出与CD 8（+）-CD 122（+）细胞相似的调节活性。虽然小鼠中存在CD 8（+）CD 122（+）CXCR 3（+）细胞，但人类中存在CD 8（+）CXCR 3（+）细胞，但不存在CD 8（+）CD 122（+）细胞。在体外试验中，人CD 8（+）CXCR 3（+）细胞显示出产生IL-10和抑制CD 8（+）CXCR 3（-）细胞产生IFN-γ的调节活性。这些结果表明，人CD 8（+）CXCR 3（+）T细胞是鼠CD 8（+）CD 122（+）Treg的对应物。
The importance of CD8(+)CD122(+) Treg in the maintenance of immune homeostasis has been previously demonstrated in mice. Because the expression pattern of CD8 and CD122 in humans is different from that in mice, human CD8(+) Treg that correspond to the murine CD8(+)CD122(+) Treg have not been identified. In this study, we performed DNA microarray analyses to compare the gene expression profiles of CD8(+)CD122(+) cells and CD8(+)CD122(-) cells in mice and found that CXCR3 was preferentially expressed in CD8(+)CD122(+) cells. When we analyzed the expression of CD122 and CXCR3 in murine CD8(+) cells, we observed a definite population of CD122(+)CXCR3(+) cells. CD8(+)CXCR3(+) cells in mice showed similar regulatory activities to CD8(+)-CD122(+) cells by in vivo and in vitro assays. While CD8(+)CD122(+)CXCR3(+) cells are present in mice, CD8(+)CXCR3(+) cells, but not CD8(+)CD122(+) cells, are present in humans. in the in vitro assay, human CD8(+)CXCR3(+) cells showed the regulatory activity of producing IL-10 and suppressing IFN-gamma production from CD8(+) CXCR3(-) cells. These results suggest that human CD8(+)CXCR3(+) T cells are the counterparts of murine CD8(+)CD122(+) Treg.