Zm401p10, encoded by an anther-specific gene with short open reading frames, is essential for tapetum degeneration and anther development in maize.
Zm401p10, encoded by an anther-specific gene with short open reading frames, is essential for tapetum degeneration and anther development in maize.
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DOI：
10.1071/fp08154
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发表时间：
2009-01
期刊：
影响因子：
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通讯作者：
Dongxue Wang;Chengxia Li;Qian Zhao;Linna Zhao;Meizhen Wang;Dengyun Zhu;G. Ao;Jingjuan Yu
中科院分区：
文献类型：
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作者：
Dongxue Wang;Chengxia Li;Qian Zhao;Linna Zhao;Meizhen Wang;Dengyun Zhu;G. Ao;Jingjuan Yu
在开花植物中，绒毡层被认为在花粉发育的早期阶段发挥着至关重要的作用。绒毡层发育和退化的破坏通常会导致雄性不育。本研究描述了玉米 (Zea mays L.) 花药特异性基因 Zm401，该基因仅包含短开放阅读框 (sORF)。 Zm401 基因的最长 ORF 编码一种称为 Zm401p10 的小蛋白质，该蛋白质在细胞核中积累。 Zm401p10 在玉米中的过度表达会延缓绒毡层退化并导致小孢子异常。微阵列分析鉴定出过表达 Zm401p10 的花药中有 278 个下调基因和 150 个上调基因。这些结果表明Zm401基因是调节玉米花药发育和雄性育性的分子网络的主要组成部分之一，并且Zm401p10从该基因的最长ORF表达。
In flowering plants, the tapetum is proposed to play a vital role in the early stages of pollen development. Disruptions to tapetum development and degeneration typically result in male sterility. The present study characterised a maize (Zea mays L.) anther-specific gene, Zm401, which only contains short open reading frames (sORFs). The longest ORF of the Zm401 gene encodes a small protein designated Zm401p10 that accumulates in the nucleus. Overexpression of Zm401p10 in maize retarded tapetal degeneration and caused microspore abnormalities. A microarray analysis identified 278 downregulated and 150 upregulated genes in anthers overexpressing Zm401p10. These results indicate that the Zm401 gene is one of the major components of the molecular network regulating maize anther development and male fertility, and that Zm401p10 is expressed from the longest ORF of the gene.