Homologous recombination between a lactococcal bacteriophage and the chromosome of its host strain
Homologous recombination between a lactococcal bacteriophage and the chromosome of its host strain
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DOI：
10.1006/viro.2000.0226
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发表时间：
2000-04-25
期刊：
影响因子：
3.7
通讯作者：
Moineau, S
中科院分区：
文献类型：
--
作者：
Bouchard, JD;Moineau, S
基因交换是噬菌体获得新特性的重要手段。乳酸乳球菌的噬菌体占据了一个特殊的生态位，即乳品厂环境，在那里它们的种群不断变化。对于导致乳球菌属细菌遗传多样性的进化机制知之甚少。在这项研究中，我们描述了两个DNA交换涉及裂解噬菌体uI 36，一个成员的P335物种，和它的L。乳酸菌寄主它们通过与宿主染色体中存在的噬菌体相关序列的同源重组而发生。由这些重组事件产生的两种突变体对噬菌体抗性机制AbiK不敏感，并且一种突变体具有减少的爆发大小以及新的复制起点。我们建议，这种类型的DNA交换与原噬菌体或残余的原噬菌体内频繁发生的P335物种的支持P335-样的细菌之间的DNA-DNA比较。(C)北京大学出版社.
Genetic exchanges constitute a significant means by which bacteriophages acquire novel characteristics. Phages of Lactococcus lactis occupy a particular niche, the dairy factory environment, where their populations are subjected to constant changes. Little is known about the mechanisms of evolution that lead to the genetic diversity of lactococcal phages. In this study, we described two DNA exchanges involving the lytic phage uI36, a member of the P335 species, and its L. lactis host. They occurred by homologous recombination with phage-related sequences present in the host chromosome. Both mutants generated by these recombination events are insensitive to the phage resistance mechanism AbiK and one has a reduced burst size as well as a new origin of replication. We propose that this type of DNA exchange with prophages or remnants of prophages occurs frequently within the P335 species as supported by DNA-DNA comparisons between P335-like phages. (C) 2000 Academic Press.