Imperatorin inhibits mitogen-activated protein kinase and nuclear factor kappa-B signaling pathways and alleviates neuroinflammation in ischemic stroke.
Imperatorin inhibits mitogen-activated protein kinase and nuclear factor kappa-B signaling pathways and alleviates neuroinflammation in ischemic stroke.
复制标题
DOI：
10.1111/cns.13748
复制
发表时间：
2022-01
影响因子：
5.5
通讯作者：
Zhu XL
中科院分区：
文献类型：
--
作者：
Ge JW;Deng SJ;Xue ZW;Liu PY;Yu LJ;Li JN;Xia SN;Gu Y;Bao XY;Lan Z;Xu Y;Zhu XL
小胶质细胞介导的神经炎症在缺血性卒中的病理过程中起重要作用，欧前胡素对卒中后神经炎症的影响尚不完全清楚。用欧前胡素处理原代小胶质细胞2小时，再加入内毒素(100 ng/ml)处理24小时，用RT-PCR、ELISA法和Western印迹法检测炎性细胞因子的表达。免疫印迹法检测MAPK和NF-κB信号通路的激活情况。采用C57BL/6J小鼠一过性大脑中动脉闭塞(TMCAO)模型确定脑缺血损伤程度。用行为学测试来评估MCAO小鼠的神经功能缺陷。TTC染色测量心肌梗死体积。欧前胡素抑制脂多糖诱导的小胶质细胞活化和促炎细胞因子的释放，减轻MCAO小鼠的缺血性损伤。转录组测序和Western印迹结果显示，下调MAPK和NF-κB通路可能参与欧前胡素的保护作用。欧前胡素下调MAPK和NF-κB信号通路，发挥抗炎作用，提示欧前胡素可能是一种潜在的治疗中风的化合物。欧前胡素抑制MAPKs和NF-κB信号通路，减轻缺血性卒中的神经炎症。
Microglia‐mediated neuroinflammation plays an important role in the pathological process of ischemic stroke, and the effect of imperatorin on post‐stroke neuroinflammation is not fully understood. Primary microglia were treated with imperatorin for 2 h followed by LPS (100 ng/ml) for 24 h. The expression of inflammatory cytokines was detected by RT‐PCR, ELISA, and Western blot. The activation of MAPK and NF‐κB signaling pathways were analyzed by Western blot. The ischemic insult was determined using a transient middle cerebral artery occlusion (tMCAO) model in C57BL/6J mice. Behavior tests were used to assess the neurological deficits of MCAO mice. TTC staining was applied to measure infract volume. Imperatorin suppressed LPS‐induced activation of microglia and pro‐inflammatory cytokines release and attenuated ischemic injury in MCAO mice. The results of transcriptome sequencing and Western blot revealed that downregulation of MAPK and NF‐κB pathways might contribute to the protective effects of imperatorin. Imperatorin downregulated MAPK and NF‐κB signaling pathways and exerted anti‐inflammatory effects in ischemic stroke, which indicated that imperatorin might be a potential compound for the treatment of stroke. Imperatorin inhibits MAPKs and NF‐κB signaling pathways and alleviates neuroinflammation in ischemic stroke.
登录
查看更多内容
丙酮酸乙酯可防止血脑屏障损伤并改善创伤性脑损伤大鼠模型的长期神经学结果。
影响因子：
5.5
作者：
Shi H;Wang HL;Pu HJ;Shi YJ;Zhang J;Zhang WT;Wang GH;Hu XM;Leak RK;Chen J;Gao YQ
通讯作者：
Gao YQ
欧前胡素对血管性痴呆大鼠细胞凋亡和突触可塑性的影响
影响因子：
4.6
作者：
Huang Y;Liao X;Wang H;Luo J;Zhong S;Zhang Z;Zhang F;Chen J;Xie F
通讯作者：
Xie F
HLA 纯合人类 iPSC 衍生神经前体的脑内移植可改善缺血性中风啮齿动物模型的行为和病理缺陷。
影响因子：
8.5
作者：
Noh JE;Oh SH;Lee S;Lee S;Kim YH;Park HJ;Ju JH;Kim HS;Huh JY;Song J
通讯作者：
Song J
DOI：
10.3390/antiox10030362
发表时间：
2021-02-27
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
Huang MH;Lin YH;Lyu PC;Liu YC;Chang YS;Chung JG;Lin WY;Hsieh WT
通讯作者：
Hsieh WT
影响因子：
8
作者：
Cai, Wei;Dai, Xuejiao;Chen, Jun
通讯作者：
Chen, Jun