Epstein-Barr virus (EBV) detection and typing by PCR: a contribution to diagnostic screening of EBV-positive Burkitt's lymphoma.
Epstein-Barr virus (EBV) detection and typing by PCR: a contribution to diagnostic screening of EBV-positive Burkitt's lymphoma.
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DOI：
10.1186/1746-1596-1-17
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发表时间：
2006-08-07
影响因子：
2.6
通讯作者：
Zalcberg IR
中科院分区：
文献类型：
--
作者：
Hassan R;White LR;Stefanoff CG;de Oliveira DE;Felisbino FE;Klumb CE;Bacchi CE;Seuánez HN;Zalcberg IR
Epstein-Barr virus (EBV) is associated to the etio-pathogenesis of an increasing number of tumors. Detection of EBV in pathology samples is relevant since its high prevalence in some cancers makes the virus a promising target of specific therapies. RNA in situ hybridization (RISH) is the standard diagnostic procedure, while polymerase chain reaction (PCR)-based methods are used for strain (EBV type-1 or 2) distinction. We performed a systematic comparison between RISH and PCR for EBV detection, in a group of childhood B-cell Non-Hodgkin lymphomas (NHL), aiming to validate PCR as a first, rapid method for the diagnosis of EBV-associated B-cell NHL. EBV infection was investigated in formalin fixed paraffin-embedded tumor samples of 41 children with B-cell NHL, including 35 Burkitt's lymphoma (BL), from Rio de Janeiro, Brazil, by in situ hybridization of EBV-encoded small RNA (EBER-RISH) and PCR assays based on EBNA2 amplification. EBV genomes were detected in 68% of all NHL. Type 1 and 2 accounted for 80% and 20% of EBV infection, respectively. PCR and RISH were highly concordant (95%), as well as single- and nested-PCR results, allowing the use of a single PCR round for diagnostic purposes. PCR assays showed a sensitivity and specificity of 96% and 100%, respectively, with a detection level of 1 EBV genome in 5,000–10,000 EBV-negative cells, excluding the possibility of detecting low-number EBV-bearing memory cells. We describe adequate PCR conditions with similar sensitivity and reliability to RISH, to be used for EBV diagnostic screening in high grade B-NHL, in "at risk" geographic regions.
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影响因子：
11.4
作者：
Sandlund, JT;Fonseca, T;Pedrosa, F
通讯作者：
Pedrosa, F
影响因子：
6.4
作者：
Klumb, CE;Hassan, R;Maia, RC
通讯作者：
Maia, RC
DOI：
10.1007/bf02899428
发表时间：
1990-01-01
期刊：
VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY
影响因子：
--
作者：
OHSHIMA, K;KIKUCHI, M;KIMURA, N
通讯作者：
KIMURA, N
影响因子：
5.4
作者：
Decker, LL;Klaman, LD;ThorleyLawson, DA
通讯作者：
ThorleyLawson, DA
DOI：
10.3109/15513819409022030
发表时间：
1994-01-01
期刊：
PEDIATRIC PATHOLOGY
影响因子：
--
作者：
DRUT, RM;DAY, S;MEISNER, L
通讯作者：
MEISNER, L