Clathrin-mediated transport: assembly required. Workshop on Molecular Mechanisms of Vesicle Selectivity.
Clathrin-mediated transport: assembly required. Workshop on Molecular Mechanisms of Vesicle Selectivity.
复制标题
网格蛋白介导的运输：需要组装。
DOI：
10.1038/sj.embor.7400249
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发表时间：
2004
期刊：
影响因子：
7.7
通讯作者：
Puertollano,Rosa
中科院分区：
文献类型：
--
作者：
Puertollano,Rosa
维持真核细胞的结构和功能组织需要将蛋白质和脂质正确靶向其最终目的地。本次研讨会的重点是介导运输中介体形成的机制，这些中介体在细胞内区室和质膜之间运输货物。运输囊泡的形成是一个高度复杂的过程，需要协调和选择性地募集脂质、跨膜蛋白及其腔内货物，以及囊泡运输和与受体室融合所需的分选机械组件。尽管已经描述了不同类型的转运囊泡，但介导质膜内吞作用的网格蛋白包被囊泡（CCV）是最具特征的，因此通常被认为是转运中间体形成的模型。最近，通过 X 射线晶体学解析了几个外壳成分结构域的结构，并鉴定了新的调节蛋白，极大地增进了我们对 CCV 形成和功能的理解。
Maintenance of the structural and functional organization of eukaryotic cells requires the correct targeting of proteins and lipids to their final destinations. This workshop focused on the mechanisms that mediate the formation of transport intermediaries, which carry cargo between intracellular compartments and the plasma membrane. The formation of transport vesicles is a highly complex process requiring the coordinated and selective recruitment of lipids, transmembrane proteins and their lumenal cargo, and the sorting machinery components that are required for vesicle transport and fusion with acceptor compartments. Although different types of transport vesicles have been described, clathrin-coated vesicles (CCVs), which mediate endocytosis from the plasma membrane, are the best characterized and are therefore often considered a model for transport-intermediary formation. Recently, the resolution of the structure of several coat-component domains through X-ray crystallography and the identification of new regulatory proteins has greatly advanced our understanding of CCV formation and function.