Growth, morphologic, and invasive characteristics of early and late passages of a human endometrial carcinoma cell line (RL95-2).
Growth, morphologic, and invasive characteristics of early and late passages of a human endometrial carcinoma cell line (RL95-2).
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人子宫内膜癌细胞系 (RL95-2) 早期和晚期传代的生长、形态和侵袭特征。
DOI：
10.1007/bf02634139
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hendrix,MJ
中科院分区：
文献类型：
--
作者：
Sundareshan,P;Hendrix,MJ
人子宫内膜腺癌连续细胞系（RL 95 -2）的两个体外传代，早期（传代<30次）和晚期（传代>200次）提供了一个有趣的模型来研究子宫内膜肿瘤的生长、形态学和侵袭特性。已显示雌激素受体阳性的早期传代具有与原发性肿瘤非常相似的特征，而雌激素受体阴性的晚期传代表现出转移表型的几个特征。与早期传代细胞相比，晚期传代细胞的血清依赖性较低，形成病灶，显示出更快的生长速率（由于其倍增时间较短），并达到更高的饱和密度。晚期传代细胞也表现出形态学改变，伴随着F-肌动蛋白分布的改变。尽管早期和晚期传代在体外侵袭测定中显示出相似的侵袭潜力，但晚期传代细胞凭借其几种转化的特征，与其早期传代对应物相比保持独特的性质。
Two in vitro passages of a human endometrial adenocarcinoma continuous cell line (RL95-2), an early (subcultured <30 times) and a late passage (subcultured >200 times) have provided an interesting model to study the growth, morphologic, and invasive properties of endometrial tumors. The early passage, which has been shown to be estrogen-receptor positive, has characteristics closely resembling a primary tumor, whereas the estrogen receptor negative late passage exhibits several features of the metastatic phenotype. Compared to the early passage cells, the late passage cells were less serum dependent, formed foci, demonstrated a faster rate of growth (due to their shorter doubling times), and attained higher saturation densities. The late passage cells also displayed an altered morphology which was accompanied by alterations in the distribution of F-actin. Even though early and late passages showed similar invasive potential in an in vitro invasion assay, the late passage cells, by virtue of their several transformed characteristics, maintain distinctive properties compared with their early passage counterparts.