Comparison of the different protocols of the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) by evaluating the eye irritation potential of surfactants
Comparison of the different protocols of the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) by evaluating the eye irritation potential of surfactants
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DOI：
10.1016/j.tiv.2021.105255
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发表时间：
2021-11-02
影响因子：
3.2
通讯作者：
Simoes Villas Boas, Maria Helena
中科院分区：
文献类型：
--
作者：
de Araujo Lowndes Viera, Luciana Madureira;Silva, Ronald Santos;Simoes Villas Boas, Maria Helena
鸡蛋测试 - 绒毛尿囊膜 (HET-CAM) 是一种有效的替代方法，用于评估化学品对眼睛刺激的可能性。该方法是唯一模拟眼睛结膜的方法，旨在通过观察纯或稀释化学品施用后立即对膜的刺激作用来半定量评估化学品对鸡蛋中鸡胚周围的绒毛尿囊膜的刺激潜力。本研究的目的是通过评估表面活性剂的眼睛刺激潜力，比较 HET-CAM 的不同方案、法国和德国方案。通过比较，我们对法国协议进行了优化，生成了一个经过调整的协议，以减少测试评估中的主观性，确保更准确的结果和更好的质量控制。比较表明，法国和德国协议中获得的结果没有显着差异。众所周知，HET-CAM 会高估结果并能够准确识别无刺激性产品，因此它是自下而上测试策略的一个很好的候选者。它还可以用于一系列测试以完全替代兔子。
The Hen's Egg Test - Chorioallantoic Membrane (HET-CAM) is a valid alternative method used to assess the potential for eye irritation from chemicals. This method is the only one that mimics the conjunctivae of the eye and aims to semi-quantitatively evaluate the irritant potential of a chemical on the chorioallantoic membrane surrounding the chicken embryo in egg by observing the irritation effects on the membrane immediately after the pure or diluted chemical is applied. The purpose of this study was to compare the different protocols of the HET-CAM, the French and German protocols, by evaluating the eye irritation potential of surfactants. The comparison led to the optimization of the French protocol, generating an adapted one, to reduce subjectivity in the test evaluation, ensuring more accurate results and greater quality control. The comparison showed that there are no significant differences between the results obtained in the French and German protocols. HET-CAM is known to overestimate the results and to be able to accurately identify non-irritant products and it is a great candidate to be part of a Bottom-up test strategy. It also can be used in a battery of tests to completely replace rabbits.