Evidence of Interdomain Ammonium Cross-Feeding From Methylamine- and Glycine Betaine-Degrading Rhodobacteraceae to Diatoms as a Widespread Interaction in the Marine Phycosphere.
Evidence of Interdomain Ammonium Cross-Feeding From Methylamine- and Glycine Betaine-Degrading Rhodobacteraceae to Diatoms as a Widespread Interaction in the Marine Phycosphere.
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DOI：
10.3389/fmicb.2020.533894
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发表时间：
2020
影响因子：
5.2
通讯作者：
Philipp B
中科院分区：
文献类型：
--
作者：
Zecher K;Hayes KR;Philipp B
溶解有机氮（DON）化合物，如甲胺（MAs）和甘氨酸甜菜碱（GBT）在海洋栖息地中以可检测的浓度存在，也由微藻产生和释放。对于许多海洋细菌来说，这些DON化合物可以作为碳，能量和氮源，但微藻通常不能代谢它们。有趣的是，以前的研究表明，Donghicola sp.菌株KarMa-海洋杜鹃花科的一员-可以交叉饲料铵，使其降解一甲胺（MMA）后产生的铵，然后作为硅藻Phaeodactylum tricornutum的氮源;因此，这些生物在光合自养条件下形成相互代谢相互作用。在本研究中，我们研究了这种相互作用是否在细菌-硅藻相互作用中发挥更广泛的作用。结果表明，菌株KarMa和三角褐指藻之间的交叉喂养也可能与二-和三甲基胺以及与GBT。此外，在以MMA为唯一氮源的共培养物中，还观察到KarMa菌株与咖啡形双眉藻和海洋海链藻的交叉摄食。关于涉及其他杜鹃花科菌株的交叉喂养，MA和GBT降解途径的计算机模拟分析表明，藻类相关的杜鹃花科型菌株可能以与KarMa菌株相似的方式与三角褐指藻相互作用。对于这些类型的菌株（如嗜盐芽孢杆菌，Roseobacter alophilus，Roseovarius indicus，Ruegeria pomeroyi和Sulfitamycartillucicola），甲胺降解后的铵交叉喂养表现出物种特异性的模式，而细菌GBT降解总是导致硅藻的生长。总的来说，DON化合物的降解的Rhododocellaceae家庭和随后的交叉喂养的铵可能代表一个广泛的，生物体特异性的，和调节代谢相互作用，建立和稳定协会与光合自养硅藻在海洋中。
Dissolved organic nitrogen (DON) compounds such as methylamines (MAs) and glycine betaine (GBT) occur at detectable concentrations in marine habitats and are also produced and released by microalgae. For many marine bacteria, these DON compounds can serve as carbon, energy, and nitrogen sources, but microalgae usually cannot metabolize them. Interestingly though, it was previously shown that Donghicola sp. strain KarMa—a member of the marine Rhodobacteraceae—can cross-feed ammonium such that the ammonium it produces upon degrading monomethylamine (MMA) then serves as nitrogen source for the diatom Phaeodactylum tricornutum; thus, these organisms form a mutual metabolic interaction under photoautotrophic conditions. In the present study, we investigated whether this interaction plays a broader role in bacteria–diatom interactions in general. Results showed that cross-feeding between strain KarMa and P. tricornutum was also possible with di- and trimethylamine as well as with GBT. Further, cross-feeding of strain KarMa was also observed in cocultures with the diatoms Amphora coffeaeformis and Thalassiosira pseudonana with MMA as the sole nitrogen source. Regarding cross-feeding involving other Rhodobacteraceae strains, the in silico analysis of MA and GBT degradation pathways indicated that algae-associated Rhodobacteraceae-type strains likely interact with P. tricornutum in a similar manner as the strain KarMa does. For these types of strains (such as Celeribacter halophilus, Roseobacter denitrificans, Roseovarius indicus, Ruegeria pomeroyi, and Sulfitobacter noctilucicola), ammonium cross-feeding after methylamine degradation showed species-specific patterns, whereas bacterial GBT degradation always led to diatom growth. Overall, the degradation of DON compounds by the Rhodobacteraceae family and the subsequent cross-feeding of ammonium may represent a widespread, organism-specific, and regulated metabolic interaction for establishing and stabilizing associations with photoautotrophic diatoms in the oceans.
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影响因子：
5.1
作者：
Grossart, Hans-Peter;Czub, Gertje;Simon, Meinhard
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1413137112
发表时间：
2015-01-13
影响因子：
11.1
作者：
Durham, Bryndan P.;Sharma, Shalabh;Moran, Mary Ann
通讯作者：
Moran, Mary Ann
影响因子：
2
作者：
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通讯作者：
Dupuy, Christine
影响因子：
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作者：
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通讯作者：
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