Physiological and transcriptional responses to salt stress in salt-tolerant and salt-sensitive soybean (Glycine max [L.] Merr.) seedlings
Physiological and transcriptional responses to salt stress in salt-tolerant and salt-sensitive soybean (Glycine max [L.] Merr.) seedlings
复制标题
耐盐和盐敏感大豆 (Glycine max [L.] Merr.) 幼苗对盐胁迫的生理和转录反应
DOI：
10.1002/ldr.3005
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发表时间：
2018
影响因子：
4.7
通讯作者：
Yu Deyue
中科院分区：
文献类型：
--
作者：
Ning Lihua;Kan Guizhen;Shao Hongbo;Yu Deyue
过量的盐分是对农业的主要环境威胁。然而，大豆（Glycine max[L.]梅尔）仍然不清楚。本研究旨在探讨耐盐和盐敏感大豆在盐胁迫下生理特性的差异以及8种阳离子反向转运蛋白的表达模式。盐胁迫对盐敏感大豆生长和光合作用的抑制作用强于耐盐大豆。在150 mM NaCl处理下，幼苗中磷酸盐和大多数其他离子的浓度显着下降。K+、Mg2+和P相对浓度与相对净光合速率呈显著正相关，GmNHX 1（Glyma. 10G158700）和GmNcl 1（Glyma. 03g171500）在耐盐和盐敏感品种中的表达模式相似。此外，这两个基因的转录水平与大豆叶片中的Na+浓度之间存在显著的相关性。除GmSALT 3基因是大豆耐盐性的主效基因外，GmNHX 1和GmNcl 1基因可能与大豆耐盐性存在一定的相关性。在盐胁迫下，耐盐品种叶片中的K+、Mg2+和P浓度高于盐敏感品种，光合速率也高于盐敏感品种。研究结果可为大豆耐盐分子育种和盐碱地的可持续利用提供理论参考。
Excess salinity is a major environmental threat to agriculture. However, the adaptive mechanisms underlying salt tolerance in soybean (Glycine max[L.] Merr.) remain unclear. The aim of this study was to investigate the differences between salt‐tolerant and salt‐sensitive soybeans in their physiological characteristics and the expression patterns of 8 cation antiporters under salt stress. The inhibitory effects of salt stress on growth and photosynthesis in salt‐sensitive soybean were stronger than those in salt‐tolerant soybean. The concentration of phosphate and most other ions in the seedlings significantly declined under a 150‐mM‐NaCl treatment. Relative K+, Mg2+, and P concentrations were significantly positively correlated with relative net photosynthetic rate.GmNHX1(Glyma.10G158700) andGmNcl1(Glyma.03g171500) had similar expression patterns in the salt‐tolerant and salt‐sensitive cultivars. Furthermore, a significant correlation was present between the transcript levels of these 2 genes and the Na+concentration in soybean leaves. In addition toGmSALT3, which is the major gene in soybean for salt tolerance in previous studies,GmNHX1andGmNcl1may be associated with salt tolerance in soybean to some extent. The salt‐tolerant cultivars in this study maintained higher K+, Mg2+, and P concentrations in the leaf and had higher photosynthetic rates than the salt‐sensitive cultivar did under salt stress. These findings can provide theoretical references for the molecular breeding of salt‐tolerant soybean and the sustainable use of saline soils.