Changes in the redox state and composition of the quinone pool of Escherichia coli during aerobic batch-culture growth
Changes in the redox state and composition of the quinone pool of Escherichia coli during aerobic batch-culture growth
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DOI：
10.1099/mic.0.2007/006098-0
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发表时间：
2007-06-01
期刊：
影响因子：
2.8
通讯作者：
de Mattos, M. J. Teixeira
中科院分区：
文献类型：
--
作者：
Bekker, M.;Kramer, G.;de Mattos, M. J. Teixeira
泛醌（UQs）和甲基萘醌（mk）在大肠杆菌中发挥着不同的功能。然而，一般来说，uq主要参与有氧呼吸，而mk在厌氧呼吸中作为电子载体，uq和mk都可以接受来自各种脱氢酶的电子，并可能向不同的氧化酶提供电子。因此，它们在维持大肠杆菌的代谢灵活性方面发挥作用，每当该生物体必须适应具有变化的氧化还原特性的条件时，例如氧的可用性。在这里，作者报告了当环境从充氧良好变为低氧可用性时，醌池的大小和氧化还原状态的变化。结果表明，这种转变伴随着去甲基萘醌库的快速增加和MK库的缓慢增加。此外，在一个摇得很好的Erlenmeyer烧瓶中的指数增长培养中，可以观察到伪稳态的假设并不适用于醌池的氧化还原状态。
Ubiquinones (UQs) and menaquinones (MKs) perform distinct functions in Escherichia coli. Whereas, in general, UQs are primarily involved in aerobic respiration, the MKs serve as electron carriers in anaerobic respiration, Both UQs and MKs can accept electrons from various dehydrogenases, and may donate electrons to different oxidases. Hence, they play a role in maintaining metabolic flexibility in E coli whenever this organism has to adapt to conditions with changing redox characteristics, such as oxygen availability. Here, the authors report on the changes in both the size and the redox state of the quinone pool when the environment changes from being well aerated to one with low oxygen availability. It is shown that such transitions are accompanied by a rapid increase in the demethylmenaquinone pool, and a slow increase in the MK pool. Moreover, in exponentially growing cultures in a well-shaken Erlenmeyer flask, it is observed that the assumption of a pseudo-steady state does not hold with respect to the redox state of the quinone pool.