Profiling gene expression during the differentiation and development of the murine embryonic gonad
Profiling gene expression during the differentiation and development of the murine embryonic gonad
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DOI：
10.1095/biolreprod.104.033696
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发表时间：
2005-02-01
影响因子：
3.6
通讯作者：
Griswold, MD
中科院分区：
文献类型：
--
作者：
Small, CL;Shima, JE;Griswold, MD
微阵列技术在哺乳动物器官发生研究中的应用可以为诱导功能性组织所需的步骤提供更多的见解。为此，产生了基因表达的时间谱，目的是鉴定发育中的雄性和雌性胚胎性腺内发生的基因表达变化。在交配后11.5、12.5、14.5、16.5和18.5天（dpc）从小鼠胚胎收集性腺组织，并使用Affyphase MGU 74 v2微阵列平台测定mRNA的相对稳态水平。统计分析产生了3693转录表现出差异表达在男性和/或女性性腺发育。在11.5 dpc时，性腺在形态上是无关紧要的，但在12.5 dpc时，通过睾丸索的出现可以辨别向雄性或雌性表型的转变。许多基因在此期间表达，许多基因在两性中具有相似的表达谱。正如预期的那样，两个众所周知的性别决定基因的表达，特别是Sry和Sox 9，是睾丸所特有的。超过12.5 dpc，差异基因表达变得越来越明显，因为男性和女性组织形态和生理上的分歧。这是明显的两个独特的转录活性波发生后14.5 dpc的男性和女性。通过这项研究，可以在整个雄性和雌性胚胎性腺发育过程中检查大量包含鼠转录组的转录物，并允许更完整地描述性腺分化和发育。
The application of microarray technology to the study of mammalian organogenesis can provide greater insights into the steps necessary to elicit a functionally competent tissue. To this end, a temporal profile of gene expression was generated with the purpose of identifying changes in gene expression occurring within the developing male and female embryonic gonad. Gonad tissue was collected from mouse embryos at 11.5, 12.5, 14.5, 16.5, and 18.5 days postcoitum (dpc) and relative steady-state levels of mRNA were determined using the Affymetrix MGU74v2 microarray platform. Statistical analysis produced 3693 transcripts exhibiting differential expression during male and/or female gonad development. At 11.5 dpc, the gonad is morphologically indifferent, but at 12.5 dpc, transitions to a male or female phenotype are discernible by the appearance of testicular cords. A number of genes are expressed during this period and many share similar expression profiles in both sexes. As expected, the expression of two well-known sex determination genes, specifically Sry and Sox9, is unique to the testis. Beyond 12.5 dpc, differential gene expression becomes increasingly evident as the male and female tissue morphologically and physiologically diverges. This is evident by two unique waves of transcriptional activity occurring after 14.5 dpc in the male and female. With this study, a large number of transcripts comprising the murine transcriptome can be examined throughout male and female embryonic gonad development and allow for a more complete description of gonad differentiation and development.