Specific region of the c-myc promoter is responsive to electric and magnetic fields.
Specific region of the c-myc promoter is responsive to electric and magnetic fields.
复制标题
c-myc 启动子的特定区域对电场和磁场有反应。
DOI：
10.1002/jcb.240540304
复制
发表时间：
1994
影响因子：
4
通讯作者：
Shirley-Henderson,A
中科院分区：
文献类型：
--
作者：
Lin,H;Goodman,R;Shirley-Henderson,A
暴露于60 Hz极低频（elf）电磁（EM）场的细胞中c‐ myc转录物的水平增加。本实验的目的是确定c-mycgene上游的调控区是否调节对EM场的反应。将小鼠和人c-mycgenes的P1上游的DNA作为CAT构建体转染到细胞中。人或小鼠myc基因5′端DNA的存在导致细胞暴露于60 Hz极低频电磁场20 min后CAT表达增加。人类上游DNA的特定部分被删除并引入细胞中。对电磁场有反应的区域位于相对于P1启动子的-353和-1，257之间。
The level of c‐myctranscripts is increased in cells exposed to extremely low frequency (elf) electromagnetic (EM) fields at 60 Hz. The aim of the present experiments was to determine if regulatory regions upstream of the c‐mycgene modulate the response to EM fields. DNA upstream of P1 of both mouse and human c‐mycgenes was transfected into cells as CAT constructs. The presence of DNA 5′ to the human or mouse myc genes results in increased expression of CAT following 20 min exposures of cells to 60 Hz elf EM fields. Specific portions of the human upstream DNA were deleted and introduced into cells. The region responsive to EM fields is located between −353 and −1,257 relative to the P1promoter.