Cell attachment area of rat mesenchymal stem cells correlates with their osteogenic differentiation level on substrates without osteoconductive property
Cell attachment area of rat mesenchymal stem cells correlates with their osteogenic differentiation level on substrates without osteoconductive property
复制标题
大鼠间充质干细胞的细胞附着面积与其在不具有骨传导特性的基质上的成骨分化水平相关
DOI：
10.1016/j.bbrc.2020.03.013
复制
发表时间：
2020
影响因子：
3.1
通讯作者：
伊藤敦夫
中科院分区：
文献类型：
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作者：
橋本祥吾;安永茉由;廣瀬志弘;欠端雅之;屋代英彦;山崎淳司;伊藤敦夫
细胞形态与增殖和分化有关。我们曾报道大鼠骨髓间充质干细胞（MSCs）在不同微结构的骨传导性羟基磷灰石（HAp）上的细胞附着面积与其成骨分化水平呈负相关。在这项研究中，细胞附着面积和成骨分化水平之间的相关性进行了研究，基板上没有骨传导性能，使用组织培养聚苯乙烯（TCPS），和3摩尔%氧化钇稳定的氧化锆（3 Y-TZP）与或不与表面周期性微结构。结果发现，培养3周后的成骨分化水平增加，而培养3小时后的细胞附着面积减少。三种基质中细胞贴壁面积与骨钙素分泌量的相关系数平方为0.845。因此，即使在没有骨传导性质的基质上培养，也证实了细胞附着面积和分化水平之间的负相关性。这些结果表明，大鼠MSCs的细胞附着面积和成骨分化水平之间的相关性也适用于各种类型的基板，无论骨传导性能。
Cell morphology is related to proliferation and differentiation. We previously reported that cell attachment area of rat mesenchymal stem cells (MSCs) is negatively correlated with their osteogenic differentiation level on osteoconductive hydroxyapatite (HAp) with various microstructures. In this study, the correlation between the cell attachment area and osteogenic differentiation level was investigated on substrates without osteoconductive property using tissue culture polystyrene (TCPS), and 3 mol% yttria-stabilized zirconia (3Y-TZP) with or without surface periodic microstructures. It was found that the osteogenic differentiation level after 3 weeks of culture increased with a decrease in cell attachment area after 3 h of culture. The square of the correlation coefficient between cell attachment area and osteocalcin secretion content was 0.845 among the three types of substrates. Thus, the negative correlation between cell attachment area and differentiation level is confirmed even when cultured on substrates without osteoconductive property. These findings suggest that the correlation between the cell attachment area of rat MSCs and osteogenic differentiation level could also apply to various types of substrate, regardless of osteoconductive property.