The involvement of neurofilament heavy chain phosphorylation in the maturation and degeneration of rat oocytes.
The involvement of neurofilament heavy chain phosphorylation in the maturation and degeneration of rat oocytes.
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DOI：
10.1210/en.2011-2026
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发表时间：
2012-02
期刊：
影响因子：
4.8
通讯作者：
Noriyuki Takahashi;B. Ishizuka
中科院分区：
文献类型：
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作者：
Noriyuki Takahashi;B. Ishizuka
神经丝（NF）是一种中间丝蛋白，最初发现在神经元中表达，并参与轴突结构的维持。然而，关于NF在其他器官中的表达及其生理意义的研究还不多。在本研究中，我们研究了NF在大鼠卵巢中的表达，发现NF重链（NF-H）表达于卵泡从初级到成熟阶段的卵母细胞，排卵卵母细胞，受精卵，和闭锁卵泡的退化卵母细胞。细胞质NF-H消失在胚胎发育的两细胞阶段，而闭锁卵泡的退化卵母细胞继续表达NF-H，即使在破碎后。特异性识别磷酸化NF-H（pNF-H）的抗体用于检查卵母细胞中NF-H磷酸化的模式。pNF-H在成熟卵泡和闭锁卵泡的卵母细胞、排卵卵母细胞和单细胞受精卵的细胞质和细胞核中均有表达。用3，3 '-亚氨基二丙腈处理，其诱导神经元胞体中NF的异常磷酸化并引起神经病，诱导卵母细胞变性伴卵泡闭锁，卵母细胞中NF-H的磷酸化和细胞周期蛋白依赖性激酶5（NF-H的候选激酶）的卵巢基因表达。然而，神经元变性的指示剂Fluoro-Jade C未能染色pNF-H免疫阳性卵母细胞。我们的研究结果表明，卵母细胞中表达的NF-H可能参与卵泡生长过程中卵母细胞结构的维持，并且在短暂卵母细胞中NF-H的磷酸化可能有助于卵母细胞的退化过程。
Neurofilaments (NF) are intermediate filament proteins that were originally found to be expressed in neurons and are involved in the maintenance of axonal structure. However, there has not been much research on the expression and physiological significance of NF in other organs. In the present study, we examined the expression of NF in rat ovaries and found that NF heavy chain (NF-H) was expressed in oocytes of follicles from the primary to mature stages, ovulated oocytes, fertilized zygotes, and degenerative oocytes of atretic follicles. Cytoplasmic NF-H disappeared at the two-cell stage of embryonic development, whereas degenerative oocytes of atretic follicles continued to express NF-H even after fragmentation. An antibody that specifically recognizes phosphorylated NF-H (pNF-H) was used to examine the pattern of NF-H phosphorylation in oocytes. pNF-H was detected in the cytoplasm and nuclei of oocytes of mature and atretic follicles, ovulated oocytes, and one-cell zygotes. Treatment with 3,3'-iminodipropionitrile, which induces aberrant phosphorylation of NF in the perikarya of neurons and causes neuropathy, induced oocyte degeneration with follicular atresia, phosphorylation of NF-H in oocytes, and ovarian gene expression of cyclin-dependent kinase 5, a candidate kinase of NF-H. However, an indicator of neuron degeneration, Fluoro-Jade C, failed to stain the pNF-H-immunopositive oocytes. Our results indicate that NF-H expressed in oocytes may be involved in the maintenance of oocyte structure during follicular growth and that the phosphorylation of NF-H in ephemeral oocytes may contribute to the degeneration process of oocytes.