MICROVILLAR CELLS OF THE OLFACTORY EPITHELIUM - MORPHOLOGY AND REGENERATION FOLLOWING EXPOSURE TO TOXIC COMPOUNDS
MICROVILLAR CELLS OF THE OLFACTORY EPITHELIUM - MORPHOLOGY AND REGENERATION FOLLOWING EXPOSURE TO TOXIC COMPOUNDS
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DOI：
10.1016/0006-8993(94)01144-7
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发表时间：
1995-01-09
期刊：
影响因子：
2.9
通讯作者：
HASTINGS, L
中科院分区：
文献类型：
--
作者：
MILLER, ML;ANDRINGA, A;HASTINGS, L
近年来，微绒毛细胞（MVC）已被确定在许多物种的嗅觉上皮，包括啮齿动物，犬，灵长类动物。然而，目前对该细胞的形态学或组织化学特征没有共识，也不知道这些细胞的功能。以前的研究已经检查了MVC在发展过程中，并在成熟的嗅上皮，但不是在中毒后的侮辱。研究了成年雄性Long-Evans大鼠的微绒毛细胞（由特定的形态学标准定义），这些大鼠通过吸入200 ppm甲基溴，每天4小时，每周4天，持续2周，或吸入635 μ g/m3镍，每天6小时，连续16天，并在几个月内连续处死。MVC的恢复模式根据嗅上皮损伤的严重程度和特异性而不同。与甲基溴，所有类型的细胞完全耗尽后立即从嗅上皮损伤，包括MVC。MVC在嗅上皮中的增殖速度较慢，且仅在嗅上皮在其他方面完整时出现。与镍暴露，其中的主要影响是逐渐减少的支持细胞的顶端细胞质厚度变薄，MVC显示在曝光过程中下降，但在恢复过程中再次出现。在这两种情况下，嗅觉功能没有差异，即使当MVC从嗅上皮缺席。一个成熟的嗅上皮似乎是必要的，以支持这种MVC的存在，这表明它是不是至关重要的再生过程或嗅觉功能的恢复，但也许发挥了一些作用，尚未确定，在未受干扰的嗅上皮。
In recent years microvillar cells (MVC) have been identified in the olfactory epithelium of numerous species, including rodents, canines, and primates. However, there is no consensus on the morphologic or histochemical features of this cell, nor is the function of these cells currently known. Previous studies have examined MVC during development and in the mature olfactory epithelium, but not after toxic insult. A microvillar cell, defined by specific morphologic criteria, was studied in adult male Long-Evans rats exposed via inhalation to either 200 ppm methyl bromide for 4 h/day, 4 days/week for 2 weeks, or to 635 mu g/m(3) nickel for 6 h/day for 16 consecutive days, and sacrificed serially over several months. The pattern of recovery for MVC differed according to the severity and specificity of the insult to the olfactory epithelium. With methyl bromide, all cell types were completely depleted from olfactory epithelium immediately after injury, including MVC. MVC were slow to repopulate the epithelium, and appeared only when olfactory epithelium was complete in other respects. With nickel exposure, where the major effect was a gradual decrease in sustentacular cells with a thinning of the apical cytoplasm thickness, MVC showed a decline during exposure, but reappeared during recovery. In both cases, there was no difference in olfactory function, even when MVC were absent from the olfactory epithelium. A mature olfactory epithelium appears to be necessary to support the presence of this MVC, suggesting that it is not crucial to the regeneration processes or recovery of olfactory function, but perhaps plays some role, as yet undefined, in the unperturbed olfactory epithelium.